半夏生物碱含量测定及止呕研究  

Determination of the total alkaloids of pinellia tuber and its anti-vomiting study in minks

作　　者：王蕾[1] 赵永娟[1] 张媛媛[2] 吴静芬[2] 张向农[2] 吉中强[2] 岳旺[1] 

机构地区：[1]青岛大学医学院药理学教研室,山东青岛266021 [2]青岛市人民医院中医科,山东青岛266001

出　　处：《中国药理学通报》2005年第7期864-867,共4页Chinese Pharmacological Bulletin

基　　金：卫生部资助项目(No20023);青岛科技局资助项目(No032NY14)

摘　　要：目的提取并测定半夏生物碱含量,研究其对水貂的止呕作用。方法提取半夏生物碱,采用酸性染料比色法测定含量;观察在顺铂(7.5mg·kg-1,ip)、阿朴吗啡(l.6mg·kg-1,sc)、硫酸铜(40mg·kg-1,ig)水貂呕吐模型的止呕作用;观察半夏生物碱对水貂运动呕吐模型(100r·min-1,1h)的影响。结果半夏生物碱含量为2.83mg·g-1,对顺铂、阿朴吗啡致水貂呕吐均有抑制作用(P<0.05),对硫酸铜及运动致水貂呕吐无效。结论半夏生物碱具止呕作用,其机制与中枢抑制有关。Aim To determine the alkaloids of pinellia tuber and to study its anti-emetic effects in minks. Method The alkaloid was tested by acid dye colorimetry; the effects of alkaloids of pinellia tuber on emesis model induced by cisplatin (7.5 mg·kg -1, ip), apomorphine(1.6 mg·kg -1, sc) or copper sulfate (40 mg·kg -1, ig) were studied; its effect on rotation-induced emesis model in minks was also observed. Results The content of alkaloids of pinellia tuber was 2.83 mg·g -1; pinellia tuber significantly inhibited the enesis model induced by cisplatin and apomorphine(P<0.05), while it could not resist the enesis induced by copper sulfate and rotation(P>0.05). Conclusion The alkaloids of pinellia tuber showed anti-emetic effect in minks. Its mechanism is probably related to its inhibiting property on central nervous system.

关 键 词：半夏 生物碱 呕吐 水貂 

分 类 号：R-332[医药卫生] R282.71