Sequence Analysis and Structural Prediction of the Severe Acute Respiratory Syndrome Coronavirus nsp5  

作　　者：Jia-HaiLU Ding-MeiZHANG Guo-LingWANG Zhong-MinGUO JuanLI Bing-YanTAN Li-PingOU-YANG Wen-HuaLING Xin-BingYU Nan-ShanZHONG 

机构地区：[1]ThePublicHealthSchoolofSunYat-SenUniversity,Guangzhou510080,China [2]GuangzhouInstituteofRespiratoryDiseases,GuangzhouMedicalCollege,Guangzhou510120,China

出　　处：《Acta Biochimica et Biophysica Sinica》2005年第7期473-479,共7页生物化学与生物物理学报（英文版）

基　　金：This research was supported by a grant from the Science Foundation for SARS of Guangdong Province (No.2003Z3-E0461)

摘　　要：The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs.Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5.In this study,bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5.Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP).The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins.The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).This study provided the foundation for further exploration of the protein's biological function,and contributed to the search for anti-SARS-CoV drugs.The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs.Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5.In this study,bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5.Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP).The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins.The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).This study provided the foundation for further exploration of the protein's biological function,and contributed to the search for anti-SARS-CoV drugs.

关 键 词：severe acute respiratory syndrome (SARS) severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) REPLICASE secondary structure 3-D structure 

分 类 号：R373.1[医药卫生—病原生物学]