REPLICASE

作品数:10被引量:9H指数:1
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相关领域:农业科学更多>>
相关作者:曹广文赵根明姜庆五陈腾郭强更多>>
相关机构:第二军医大学复旦大学中国科学院上海生命科学研究院上海第二医科大学健康科学中心更多>>
相关期刊:《Science Bulletin》《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》《Science China(Life Sciences)》《Acta Pharmacologica Sinica》更多>>
相关基金:国家自然科学基金国家重点基础研究发展计划中国博士后科学基金更多>>
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Corrigendum to“Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp,nonstructural proteins p16 and p23,playing a role in virus replication”<[Virologica Sinica 37(2022)48-59]>
《Virologica Sinica》2023年第3期480-481,共2页Jie Zhu Qiuhong Miao Hongyuan Guo Aoxing Tang Dandan Dong Jingyu Tang Fang Wang Guangzhi Tong Guangqing Liu 
Due to our negligence,the original version of this article,published online on 12 January 2022,contained a mistake in Fig.4A.The lane ofβ-actin in Western blotting was misused.The correct Fig.4 is given below.We apol...
关键词:rabbit HEMORRHAGIC Cor 
The replicase protein nsp2 of Chinese highly pathogenic porcine reproductive and respiratory virus is involved in protective immunity by promoting viral clearance
《One Health Advances》2023年第1期78-93,共16页Can Kong Dan Li Yanxin Hu Peng Gao Yongning Zhang Lei Zhou Xinna Ge Xin Guo Jun Han Hanchun Yang 
supported by the National Natural Science Foundation of China(32025035);China Agriculture Research System of MOF and MARA(CARS-35).
The genome segment for replicase protein nsp2 represents the fastest evolving region of porcine reproductive and respiratory syndrome virus(PRRSV),and our previous studies have shown that the PRRSV nsp2 genetic variat...
关键词:PRRSV NSP2 Protective immunity 
Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication被引量:1
《Virologica Sinica》2022年第1期48-59,共12页Jie Zhu Qiuhong Miao Hongyuan Guo Aoxing Tang Dandan Dong Jingyu Tang Fang Wang Guangzhi Tong Guangqing Liu 
sponsored by Shanghai Sailing Program(20YF1457700);the National Natural Science Foundation of China(32000109 and 31672572);the China Postdoctoral Science Foundation(2019M660885 and 2021T140718)
Rabbit hemorrhagic disease virus(RHDV)is a member of the Caliciviridae family and cannot be propagated in vitro,which has impeded the progress of investigating its replication mechanism.Construction of an RHDV replico...
关键词:Rabbit hemorrhagic disease virus(RHDV) NUCLEOLIN Virus replication RNA-dependent RNA polymerase(RdRp) 
Application of a recombinant replicase to localize the Trionyx sinensis hemorrhagic syndrome virus and evaluate its effects on antiviral genes of T.sinensis被引量:1
《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2021年第4期295-304,共10页Sunjian LYU Xuemei YUAN Li LIU Haiqi ZHANG Zhe YU Xiaoying HANG Weida SHI Yinglei WU 
supported by the Zhejiang Provincial Science and Technology Program(Nos.2020YSZX003 and 2020YSZX010);the Zhejiang Provincial Science Exploratory Program(No.2019TSX01),China。
Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV)is an arterivirus newly discovered in Chinese softshell turtles.Little is known about the effect of antibodies against the virus or the distribution of the virus in di...
关键词:Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV) REPLICASE Virus localization Immune genes 
RNAi-Mediated Resistance to Cucumber Mosaic Virus (CMV) in Genetically Engineered Tomato
《American Journal of Plant Sciences》2014年第5期554-572,共19页Valentine Otang Ntui Kynet Kong Pejman Azadi Raham Sher Khan Dong Poh Chin Tomoko Igawa Masahiro Mii Ikuo Nakamura 
Cucumber mosaic virus is one of the most constraints to the production of tomato and other vegetable crops worldwide. Here, we generated an RNAi construct containing inverted repeat of 1138 bp fragment of a partial re...
关键词:CMV dsRNA PTGS REPLICASE Gene RNAI TOMATO 
Sequence Analysis and Structural Prediction of the Severe Acute Respiratory Syndrome Coronavirus nsp5被引量:1
《Acta Biochimica et Biophysica Sinica》2005年第7期473-479,共7页Jia-HaiLU Ding-MeiZHANG Guo-LingWANG Zhong-MinGUO JuanLI Bing-YanTAN Li-PingOU-YANG Wen-HuaLING Xin-BingYU Nan-ShanZHONG 
This research was supported by a grant from the Science Foundation for SARS of Guangdong Province (No.2003Z3-E0461)
The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs.Few studies have been conducted into the function of the severe acute respirator...
关键词:severe acute respiratory syndrome (SARS) severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) REPLICASE secondary structure 3-D structure 
Putative caveolin-binding sites in SARS-CoV proteins
《Acta Pharmacologica Sinica》2003年第10期1051-1059,1063,1064,共11页蔡全才 姜庆五 赵根明 郭强 曹广文 陈腾 
Project supported by the Key Programs of oppugning SARS from the Ministry of Education of China (№ 10) the special programs of oppugning SARS from the Shanghai Science and Technology Commission (№ NK2003-002).
AIM: To obtain the information of protein-protein interaction between the SARS-CoV proteins and caveolin-1, identify the possible caveolin-binding sites in SARS-CoV proteins. METHODS: On the basis of three related cav...
关键词:severe acute respiratory syndrome (SARS) caveolin-binding motif replicase 1AB spike protein M protein BIOINFORMATICS molecular modeling molecular docking 
Mutation analysis of 20 SARS virus genome sequences: evidence for negative selection in replicase ORF1b and spike gene
《Acta Pharmacologica Sinica》2003年第8期741-745,共5页胡兰靛 郑广勇 江海松 夏煜 张艺 孔祥银 
Project supported by National High Technology "863" Programs of China;National Natural Science Foundation of China;National Science Fund for Distinguished Young Scholars, State Key Technologies R&D programme (973).
AIM: Recently, more SARS-CoV virus genome sequences are released to the GenBank database. The aim of this study is to reveal the evolution forces of SARS-CoV virus by analyzing the nucleotide mutations in these sequen...
关键词:severe acute respiratory syndrome SARS-CoV virus negative selection replicase polyprotein spike protein 
The pathogenicity on legumes of Cucumber mosaic virus was determined by 243 nucleotides on 2a polymerase gene of viral RNA2被引量:5
《Chinese Science Bulletin》2002年第9期748-750,794,共4页TAO Xiaorong ZHOU Xueping LI Guixin YU Jialin 
This work was supported by the National Key Basic Research and Development Program (Grant No. G2000016204), the Teaching and Research Award Program for Outstanding Teachers in Higher Education Institutes of Ministry of Education (MOE) ;the National Outs
We had isolated and identified two Cucumber mosaic virus (CMV) isolates, the CMV red bean (CMV-RB) isolate and the CMV pea (CMV-P1) isolate. CMV-RB induces necrotic local lesions on inoculated leaves of broad bean, pe...
关键词:CUCUMBER MOSAIC VIRUS legumes REPLICASE gene patho-genicity. 
Effects of mutated replicase and movement protein genes on attenuation of tobacco mosaic virus被引量:1
《Science China(Life Sciences)》2001年第6期628-636,共9页杨恭 邱并生 魏军亚 刘广超 
the National Natural Science Foundation of China.
Our previous reports showed that one opal mutation (UGA) and one ochre mutation (UAA) respectively located in the replicase and movement protein (MP) genes of the attenuated tomato mosaic virus K(ToMV-K) contribute to...
关键词:tobacco mosaic virus (TMV) REPLICASE movement protein (MP) ATTENUATION mutation. 
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