Putative caveolin-binding sites in SARS-CoV proteins  

SARS病毒蛋白中caveolin推测的结合位点(英文)

作　　者：蔡全才[1] 姜庆五[1] 赵根明[1] 郭强[2] 曹广文[3] 陈腾[4] 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]第二军医大学卫生勤务学系,上海200433 [3]第二军医大学卫生勤务学系流行病学教研室,上海200433 [4]第二军医大学附属长征医院

出　　处：《Acta Pharmacologica Sinica》2003年第10期1051-1059,1063,1064,共11页中国药理学报（英文版）

基　　金：Project supported by the Key Programs of oppugning SARS from the Ministry of Education of China (№ 10) the special programs of oppugning SARS from the Shanghai Science and Technology Commission (№ NK2003-002).

摘　　要：AIM: To obtain the information of protein-protein interaction between the SARS-CoV proteins and caveolin-1, identify the possible caveolin-binding sites in SARS-CoV proteins. METHODS: On the basis of three related caveolin-binding motifs, amino acid motif search was employed to predict the possible caveolin-1 related interaction domains in the SARS-CoV proteins. The molecular modeling and docking simulation methods were used to confirm the interaction between caveolin-1 and SARS-CoV proteins. RESULTS: Thirty six caveolin-binding motifs in the SARS-CoV proteins have been mapped out using bioinformatics analysis tools. Molecular modeling and simulation have confirmed 8 caveolin-binding sites. These caveolin-binding sites located in replicase 1AB, spike protein, orf3 protein, and M protein, respectively. CONCLUSION: Caveolin-1 may serve as a possible receptor of the SARS-CoV proteins, which may be associated with the SARS-CoV infection, replication, assembly, and budding.目的:获得SARS冠状病毒蛋白与caveolin-1蛋白相互作用的信息,识别病毒蛋白中可能的caveolin-1结合位点,为SARS冠状病毒蛋白的功能研究以及设计抗SARS病毒的药物 和疫苗提供线索。方法:基于3个caveolin结合基序,采用氨基酸基序搜索方法预测SARS冠状病毒蛋白中可能与caveolin-1相互作用的区域,并用分子模拟和分子对接的方法进一步证实它们之间的相互作用。结果:通过生物信息学分析,在SARS冠状病毒蛋白中发现了36个caveolin结合基序,经过分子模拟和分子对接,获得了SARS冠状病毒蛋白与caveolin-1相互作用的8个结合位点,这些caveolin-1结合位点分别位于replicase 1AB、S蛋白、ofr3蛋白和M蛋白。结论:caveolin-1可能是SARS冠状病毒蛋白结合的一个靶点,它们之间的相互作用可能与SARS冠状病毒的感染、复制、装配和释放有关。

关 键 词：severe acute respiratory syndrome (SARS) caveolin-binding motif replicase 1AB spike protein M protein BIOINFORMATICS molecular modeling molecular docking 

分 类 号：R373[医药卫生—病原生物学]