小鼠MHCⅠ类分子限制性MAGE-3多肽表位筛选  

Screening and Identification of Murine MAGE-3 Derived MHC ClassⅠ Restricted Peptide Epitope

作　　者：李强[1] 张轶[1] 陈雪华[1] 顾琴龙[1] 朱正纲[1] 刘炳亚[1] 

机构地区：[1]上海第二医科大学瑞金医院普外科上海消化外科研究所,上海200025

出　　处：《上海第二医科大学学报》2005年第7期657-660,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金(30170915)资助项目.

摘　　要：目的筛选小鼠MAGE-3抗原来源的MHCⅠ类分子限制性多肽表位。方法用计算机模拟设计,从MAGE-3中选取得分最高的5个侯选表位。根据诱导T淋巴细胞增殖能力、对肿瘤细胞的特异性杀伤活性及释放细胞因子含量,评价多肽的免疫原性及免疫反应性。结果树突状细胞(DC)负载MAGE-341~49能够强效诱导T淋巴细胞增殖,DC负载MAGE-341~49诱导的T细胞以MHCⅠ类分子限制性方式,特异性识别表达MAGE-3抗原的肿瘤细胞。DC负载MAGE-341~49诱导的T细胞群主要为CD8+T细胞,同时天然免疫系统中的NKT、γ/δT也得到活化。结论从小鼠MAGE-3抗原中筛选出CD8+T细胞识别的、MHCⅠ类分子限制性多肽表位MAGE-341~49。ObjectiveTo screen murine MAGE-3 derived MHC class restricted peptide epitope.Methods(We used) a MHC class epitope prediction algorithm to select five candidate peptides according to the score. The immunogenicity and immune reactivity of the peptide were evaluated on the basis of T-cell proliferation assay, specific lysis of tumor celLs and cytokines releasing.ResultsDC loaded with MAGE-3_(41~49) elicited potent T cells proliferation, and MAGE-3_(41~49) pulsed DC activated T cells specifically lysed MAGE-3 expressing tumor cells in the MHC class restricted manner. MAGE-3_(41~49) pulsed DC induced T cells population was mainly CD8^+T cells, and NKT, γ/T in the innate immnue system was activated concomitantly.ConclusionMAGE-3_(41~49) was identified as MHC class restricted epitope and could stimulate CD8^+T cells responses.

关 键 词：MAGE-3 抗原表位 T淋巴细胞 树突状细胞 

分 类 号：R730.3[医药卫生—肿瘤]