β-紫罗兰酮抑制SGC-7901细胞潜在的转移作用  

Inhibitory effect of potential metastasis in SGC-7901 cells induced by β-ionone

作　　者：刘家仁[1] 陈炳卿[1] 杨艳梅[1] 孙向荣[1] 董宏伟[1] 王琪[1] 杨宝峰[2] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150086 [2]哈尔滨医科大学药理学教研室

出　　处：《卫生研究》2005年第4期435-438,共4页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30200229);黑龙江省及中国博士后基金资助项目

摘　　要：目的观察β紫罗兰酮对人胃腺癌细胞(SGC7901)潜在转移的影响。方法采用细胞生长曲线,酶谱法和RTPCR技术,我们检查了不同浓度(25、50、100和200μmolL)β紫罗兰酮对SGC7901细胞生长,IV明胶酶的活性(MMP2和MMP9),nm23H1基因和金属蛋白酶抑制剂(TIMP1和TIMP2)在SGC7901细胞表达情况。β紫罗兰酮处理时间为24小时和48小时。结果β紫罗兰酮可抑制SGC7901细胞增殖。用不同浓度的β紫罗兰酮处理SGC7901细胞8天的抑制率分别为25.93%、28.21%、74.36%和90.11%。β紫罗兰酮作用于SGC7901细胞的IC50值为89μmolL。β紫罗兰酮不影响SGC7901细胞的基质金属蛋白酶2(MMP2)和基质金属蛋白酶9(MMP9)的活性。然而,β紫罗兰酮可明显地促进nm23H1基因、TIMP1和TIMP2的表达,呈现剂量-反应关系。结论β紫罗兰酮可抑制SGC7901细胞的生长和增殖。可能通过上调nm23H1,TIMP1和TIMP2来影响SGC7901细胞潜在的转移。然而,β紫罗兰酮对SGC7901细胞的IV型明胶酶活性没有影响,因而,β紫罗兰酮抑制SGC7901细胞的转移需要进一步研究。Objective To determine the effect of β-ionone on the potential metastasis of human gastric adenocarcinoma cell line SGC-7901 and the underlying mechanism.Methods Using curve of cellular growth, Zymograms, and RT-PCR assays, we analyzed the growth rate, the activities of two types IV collagenase of Matrix met alloproteinase 9 ~MMP-9) and MMP-2 and the expression of nm23-H1 gene, tissue inhibitor of met alloproteinase 1 ~TIMP-1) and TIMP-2 in SGC-7901 cells which were treated with progressively increasing concentrations ~25, 50, 100 and 200 μmol/L) of β-ionone for 24 h and 48 h.Results The growth of SGC-7901 cells was inhibited by β-ionone. Eight days after treatment with different concentrations of β-ionone, as mentioned above, the inhibition rates were 25.93%, 28.21%, 74.36% and 90.11%, respectively compared to the negative control. The estimated IC_(50) value of β-ionone for SGC-7901 cells was estimated to be 89 μmol/L; β-ionone did not show any effect on the activities of MMP-9 and MMP-2 in SGC-7901 cells. However, the expression of nm23-H1, TIMP-1 and TIMP-2 mRNA transcripts gradually increased in response to β-ionone in a dose-dependent manner. Conclusion β-ionone can inhibit the growth and proliferation of SGC-7901 cells. It may show some effects on the potential metastasis of SGC-7901 cells indicates by its upregulation of nm23-H1, TIMP-1 and TIMP-2 expression. However, β-ionone may have no effect on the activities of type IV collagenase in SGC-7901 cells. The mechanism by which β-ionone inhibits the potential metastasis of SGC-7901 cells needs to be studied further.

关 键 词：Β-紫罗兰酮 SGC-7901细胞 潜在的转移作用 

分 类 号：R730.1[医药卫生—肿瘤] R735.2[医药卫生—临床医学]