水禽流感病毒分离株A/Duck/Yangzhou/233/02(H6N2)膜蛋白基因遗传进化分析  

Phylogenetic analysis of the surface glycoprotein genes of an aquatic bird origin influenza virus isolate A/Duck/Yangzhou/233/2002(H6N2)

作　　者：张评浒[1] 刘晓文[1] 钱忠明[1] 唐应华[1] 刘秀梵[1] 薛峰[1] 郝贵杰[1] 

机构地区：[1]扬州大学,农业部畜禽传染病学重点开放实验室,扬州,225009

出　　处：《微生物学报》2005年第4期491-495,共5页Acta Microbiologica Sinica

摘　　要：采用常规的血清学收验和特异性RT-PCR方法对华东地区家养水禽中流感病毒的带毒状况进行两年多的监测,分离鉴定出多株H6亚型禽流感病毒。对其中的一株A/Duck/Yangzhou/233/02(H6N2)(简称DkYZ23302)(H6N2)的表面膜蛋白基因进行了序列测定,并与GenBank中收录的其它序列进行了比较,遗传进化结果表明DkYZ23302的血凝素基因(HA)与近年香港分离的鸭源毒株DkHK346199(H6N1)、中国台湾鸡源毒株CkTaiwanna398的亲缘关系最近;而神经氨酸酶基因(NA)遗传进化分析结果表明DkYZ23302(H6N2)的NA基因起源于禽源H9N2亚型流感病毒,这可能是不同亚型禽流感病毒在水禽体内发生基因重配的结果。DkYZ23302(H6N2)的HA推导的氨基酸剪切位点序列为P-Q-I-E-T-R-D,为典型低致病性禽流感病毒的特征序列,与对SPF鸡的致病力试验相吻合。Several H6 subtype avian A influenza viruses were isolated from aquatic birds in some live bird markets when we surveyed the ecology of the influenza in East China for more than two years and identified by specific RT-PCR. In this paper, the hemagglutinin (HA) and neuraminidase (NA) gene of one representative virus named A/Duck/Yangzhou/233/2002 (H6N2) (Dk/YZ/233/02) had been sequenced. Phylogenetic analysis of the H6 gene sequences available in the Influenza Sequence Database showed that the Dk/YZ/233/02 viruse cluster together with Dk/HK/3461/99 (H6N1) and Ck/Taiwan/na3/98 (H6N1). Phylogenetic analysis of the N2 NA genes of Dk/YZ/233/02 revealed that the NA gene of Dk/YZ/233/02 had genetically close relationships with that of H9N2 viruses isolated from duck in Japan and from chickens in South Korea, which were distinct from those of Ck/Beijing/94 (H9N2). The sequence of cleavage site between HA1 and HA2 of Dk/YZ/233/02 is P-Q-I-E-T-R-D, which was the typical characterization of the LPAIV.

关 键 词：病毒分离株 进化分析 基因遗传 低致病性禽流感病毒 RT-PCR方法 GENBANK 神经氨酸酶基因 H9N2亚型 遗传进化 膜蛋白基因 血凝素基因 致病力试验 华东地区 分离鉴定 序列测定 亲缘关系 中国台湾 NA基因 分析结果 特征序列 

分 类 号：S852.659.5[农业科学—基础兽医学]