N^+注入选育黑曲霉益生菌及其突变菌株产酶条件的研究  

Study on breeding of probiotic Aspergillus nigre by N^+ implantation and fermentation condition of its mutant

作　　者：程茂基[1] 陈丽娟[1] 蔡克周[1] 余增亮[2] 张束清[2] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]中国科学院等离子物理研究所,合肥230031

出　　处：《微生物学报》2005年第4期534-538,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30100134)~~

摘　　要：以益生菌株黑曲霉AN01为材料,经N+多次诱变得突变益生菌株AN03。结果表明,出发益生菌株AN01酸性蛋白酶、纤维素酶和果胶酶的酶活分别由原来的71.6Ug、141.7Ug和264.8Ug相继提高到996.5Ug、940.4Ug和906.5Ug。突变益生菌株AN03经传5代培养,产酶特性稳定。试验还研究了变突变益生菌株AN03最佳产酶条件,培养基为每升含麸皮105g,玉米芯105g,豆粕105g,氯化铵16g,pH5.0。30℃培养4d。Using Aspergillus niger AN01 as original strain, mutated strain Aspergillus nigerAN03 was obtained by N + implantation. The results showed that activities of acidic protease, cellulase and pectinase of Aspergillus niger were raised from 71.6U/g, 141.7U/g and 264.8U/g to 996.5U/g, 940.4U/g and 906.5U/g respectively. Characteristics of enzymes production in the mutant Aspergillus niger AN03 kept stabile by 5 times subculture.In addition, the optimum conditions for enzymes production of Aspergillus niger AN03 were investigated. The optimum components of medium consisted of bran 105.0g, corn straw 105.0g, bean cake 105.0g, NH-4Cl 6.0g, H-2O 1000mL.The optimum fermentation condition was incubated for 4d in the condition of pH 5.0 and temperature 30℃.

关 键 词：离子注入 益生菌 诱变育种 复合酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]