黄芪对柯萨奇B_3病毒感染培养大鼠心肌细胞Ca^（2＋）内流及该病毒RNA复制  

Effect of Astragalus membranaceus on Ca￣(2+) Influx and Coxsackie Virus B_3 RNA Replication in Cultured Neonatal Rat Heart Cells

作　　者：郭棋[1] 彭天庆[1] 杨英珍[1] 顾全保 赵剑星 

机构地区：[1]上海医科大学中山医院,中国科学院上海细胞生物学研究所

出　　处：《中国中西医结合杂志》1995年第8期483-485,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金

摘　　要：本研究结果发现，黄芪对柯萨奇Ｂ_３病毒（ＣＶＢ_３）感染大鼠心肌细胞及正常对照心肌细胞的Ｃａ^（２＋）内流均呈显著抑制作用（ｐ＜０．０１，Ｐ＜０．０５））；若在病毒感染１ｈ后加黄芪，经４８ｈ培养，对正常对照及感染细胞的Ｃａ^（２＋）内流亦有抑制作用（Ｐ＜０．０５）；同时细胞中ＣＶＢ_３－ＲＮＡ含量显著少于病毒对照组（Ｐ＜０．００１）。提示黄芪具有钙拮抗作用，可减少病毒感染引起的心肌Ｃａ^（２＋）内流量，有可能减轻感染细胞的继发性Ｃａ^（２＋）损伤；且对细胞中病毒ＲＮＡ的复制具有抑制作用，显示了其在病毒性心肌炎临床治疗上的应用价值。The effect of Astrapalus membranaceus(AM) on Ca2+ influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replication in cultured neonatal rat heart cells infected with CVB3 was investigated.It was found that the Ca2+ influx could be inhibited significantly(P<0.01) by AM after infection of heart cells for 48 h.In addition,when the cultured heart cells infected with CVB3 and treated with AM for 48 h,the Ca2+ influx of infected heart cells also could be inhibited by AM(P<0.05) and the amounts of CVB3-RNA in myocytes were significantly decreased than that in infected control group(P<0.001).These phenomena suggested that AM could exert the effects of decreasing the secondary Ca2+ damages,and improving the abnormal myocardial electric activity,and inhibiting replication of CVB3-RNA in myocardium.Thus, it is a rational choice to treat patients with AM in viral myocarditis.

关 键 词：心肌炎 病毒性心肌炎 黄芪 钙内流 CVB3 

分 类 号：R259.422.1[医药卫生—中西医结合]