磷脂酶C对血小板粘附性及表面膜GpIb的影响  

Effect of PLC on platelet's adhesion and GpIb

作　　者：王常高[1] 瞿永华[2] 陈涛[3] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]武汉工业学院医学院,湖北武汉430023 [3]中国科学院武汉病毒研究所,湖北武汉430071

出　　处：《西北农林科技大学学报（自然科学版）》2005年第7期5-8,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：湖北省自然科学基金项目(2004ABA233)

摘　　要：采用旋转玻球法测定了磷脂酶C(PLC)经十二指肠给药后对家兔血小板粘附性的影响,同时利用竞争性酶联免疫法测定了PLC对人血小板表面膜糖蛋白Ib(GpIb)分子数的影响。结果表明,PLC冻干辅料及100和200U/kg的PLC对家兔血小板粘附率无明显影响,而400～1000U/kgPLC对血小板粘附率具有明显的影响。PLC冻干辅料对人血小板表面膜GpIb分子数没有明显影响,而每mL血浆(PRP)中加入0.5,1.0和2.0U的PLC对人血小板表面GpIb分子数可产生明显影响。The rounding glass ball method was taken to study the effect of phospholipase C (PLC) on platelet's adhesion administered via duodenum,and competitive enzyme-linked immunosorbent assay (CELISA) was taken to study the effect of PLC on platelet's GpIb molecular number at the same time.The result was that freeze-drying additive,100 and 200 U/kg PLC,had no evident effect on platelet's adhesion rate,but 400,600,800 and 1 000 U/kg PLC had significant effect.Freeze drying additive had no apparent effect on platelet's GpIb molecular number,but 0.5,1 and 2 U/mL PRP PLC had significant effect.

关 键 词：磷脂酶C 血小板 粘附率 GPIB 

分 类 号：R973.2[医药卫生—药品]