大肠杆菌内毒素体外诱生肿瘤坏死因子及影响因素的分析  

The Study of LPS-induced TNF Cytotoxicity and the Influential Factors in vitro

作　　者：秦明放[1] 吴咸中[1] 张文治[1] 庞智玲 

机构地区：[1]天津市南开医院天津市急腹症研究所,天津医学院内分泌研究所

出　　处：《中国肿瘤临床》1995年第11期776-779,共4页Chinese Journal of Clinical Oncology

摘　　要：本实验应用细胞培养及细胞毒生物测定法分别观察了各因素（单独或组合后）对培养上清中肿瘤坏死因子（ＴＮＦ）细胞毒性的诱生作用。结果表明：经不同浓度ＬＰＳ刺激后小鼠腹腔巨噬细胞培养上清对Ｌ９２９靶细胞具有强烈的细胞毒性（Ｐ＜０．０１）。不同浓度的庆大霉素也表现出一定的ＴＮＦ诱生作用（Ｐ＜０．０５）。当各因素分别与ＬＰＳ配伍后发现，低浓度的氢化考的松便可对ＬＰＳ诱生ＴＮＦ产生抑制（Ｐ＜０．０５），随着浓度增高抑制性加强（Ｐ＜０．０１）。关于激活或抑制效应产生的机理尚有待于深入研究。Murine L929 fibroblast bioassay was used to study the change of TNF cytotoxicity in culture supernatant of murine peritoneal macrophages induced by lipopolysaccharide which was cultured with single or combination of drugs. The supernatant of macrophagesstimulated by LPS of varied concentrations demon strated strong cytotoxicity to L929 cell line (P<0.01).Gentamycin of different concentrations also was able to induce TNF of varied degree (P<0. 05).By combination with various drugs individually with LPS,it was shown that hydrocortisone in a low concentration had an inhibition effect on LPS-induced TNf secrstion (P<0.01) and this inhibition was dose dependent. The exact mechanisms of the effects warrant further study.

关 键 词：内毒素 肿瘤坏死因子 巨噬细胞 大肠杆菌 

分 类 号：R730.231.3[医药卫生—肿瘤] R730.3[医药卫生—临床医学]