^(125)I粒子近距离照射对BEL-7402肝癌细胞增殖和细胞周期的影响  

Effects of ^(125)I Seed Brachytherapy on Proliferation and Cell Cycle Distribution in BEL-7402 Cell Line

作　　者：邵文博[1] 宋金龙[2] 

机构地区：[1]山东大学齐鲁医院核医学科,山东济南250012 [2]山东省肿瘤医院外二科,山东济南250117

出　　处：《中国医学物理学杂志》2005年第4期567-567,563,共2页Chinese Journal of Medical Physics

摘　　要：目的:观察125I粒子近距离照射对BEL-7402肝癌细胞增殖和细胞周期的影响。方法:用四唑盐(MTT)法检测125I粒子近距离照射对BEL-7402细胞增殖的影响;流式细胞术检测125I粒子近距离照射对BEL-7402细胞周期的影响。结果:1、125I粒子近距离照射48、72、96小时后BEL-7402细胞增殖抑制率分别为(16.72±3.23)%、(26.44±4.71)%、(36.60±7.14)%;2、125I粒子近距离照射48小时后BEL-7402细胞周期位于G0-G1、S、G2-M期的比率分别为(40.47±0.64)%、(38.18±0.91)%、(21.35±0.65)%,而对照组分别为(54.47±1.17)%、(35.83±0.41)%、(9.71±1.27)%。结论:125I粒子近距离照射可抑制BEL-7402细胞增殖,且随剂量累积抑制率增加,125I粒子近距离照射可改变BEL-7402细胞周期分布,阻滞细胞于G2-M期。Objective To evaluate the effect of 125I seed brachytherapy on proliferation and cell cycle distribution in BEL-7402 cell line. Methods The proliferation of BEL-7402 cell was measured after 125I seed brachytherapy using MTT method. The effect of 125I seed brachytherapy on cell cycle distribution was investigated by FCM. Results The proliferation inhibition rates after 125I seed brachytherapy for 48h72h and 96h were(16.72±3.23)%(26.44±4.71) % and (36.60±7.14)%. The distribution rates of cell cycle in G0-G1SG2-M phase after 125I seed brachytherapy for 48h were (40.47±0.64)%(38.18±0.91)% and(21.35±0.65)%while in the controller were(54.47±1.17)%(35.83±0.41)% and (9.71 ±1.27)%respectively. Conclusion 125I seed brachytherapy can inhibit proliferation and the inhibition rate is related with total dose. 125I seed brachytherapy can change cell cycle distribution and cause G2-M phase arrest in BEL-7402 cell line.

关 键 词：^125I粒子 距离治疗 BEL-7402肝癌细胞 细胞增殖 细胞周期 

分 类 号：R730.55[医药卫生—肿瘤]