肿瘤浸润性淋巴细胞和肿瘤坏死因子α协同抗肿瘤作用的研究  

Combined antitumor efrect of tumor-infiltrating lymphocytes and tumor necrosis factor alpha

作　　者：殷澄[1] 张滨学 郑杰[1] 吴秉铨[1] 王洁良[1] 由江峰[1] 崔湘林 

机构地区：[1]北京医科大学病理学教研室

出　　处：《中华病理学杂志》1995年第3期162-164,共3页Chinese Journal of Pathology

基　　金：国家自然科学基金

摘　　要：利用体外消化结合不连续密度梯度离心的方法，由肿瘤组织内分离出肿瘤浸润性淋巴细胞（ＴＩＬ），并在重组人类白细胞介素２（ｒＩＬ－２）和／或重组肿瘤坏死因子α（ｒＴＮＦα）存在下进行体外培养。结果表明，低浓度ｒＴＮＦａ可以促进ＴＩＬ体外增殖、增加处于Ｓ期及Ｇ＿２＋Ｍ期的ＴＩＬ比例、增加细胞毒Ｔ细胞比例、上调ＴＩＬＩＬ－２Ｒ表达，并能明显增强ＴＩＬ体内外对自体及同种异体肿瘤的杀伤活性。umor-infiltrating lymphocytes(TILs ) were isolated from 8 solid tumors by means of in vitro di-gestion a nd d iscontinuous density grad ient centrifugation。 By incubating TILs in the presence of 6000Iu/ml rlL-2 and 500u/ml rTNFa,It’s demonstrated that rTFNa could enhance the proliferation ofTILs,promote the proportion of TILs in S-phase and G2 M-phase,enhance the percentage of CD3 +and CD8 + cells,up-regulate the expression of IL-2R,as well as enhance the cytotoxicity of TILs a-gainst autoIogous and allogenic tumors both in vitro and in vivo. The results are of significance for in-creasing immunotherapy effects and decreasing the adverse effects of certain cytokines.

关 键 词：淋巴细胞 肿瘤浸润 肿瘤坏死因子 抗肿瘤作用 

分 类 号：R730.51[医药卫生—肿瘤] R730.3[医药卫生—临床医学]