增殖细胞核抗原表达与AgNORs图像分析在判断肺癌预后上的应用  

PCNA expression and AgNORs image analysis as factors in judging the prognosis of lung cancer

作　　者：王恩华[1] 刘闺男[1] 赫明昌[1] 

机构地区：[1]中国医科大学病理学教研室,中国医科大学第一临床学院内科

出　　处：《中华病理学杂志》1995年第3期143-145,共3页Chinese Journal of Pathology

摘　　要：应用抗增殖细胞核抗原（抗ＰＣＮＡ）单克隆抗体，采用Ｓ－Ｐ免疫组化方法和ＡｇＮＯＲｓ染色图像分析，对有确切随访结果的８６例原发性肺癌和１０例肺炎性假瘤进行了研究。结果表明：经ＰＣ－ＮＡ阳性反应计算出的增殖指数（ＰＩ）与图像分析的ＡｇＮＯＲｓ计数及其颗粒总面积间存在着明显的相关性。二者均与肺癌分型有关，并随癌分化程度的增高而减小；与ＴＮＭ分期中的淋巴结（Ｎ）和远隔转移（Ｍ）有关；术后存活５年以上者明显小于３年以内者（Ｐ＜０．０１）。认为ＰＣＮＡ的表达和ＡｇＮＯＲｓ图像分析技术有助于肺癌的分型、分化及预后的判断。The expression of PCNA and AgNORs count or AgNORs’area in 86 cases of primary lung cancer and l0 cases of inflammatory pseudotumor were studied by S-P immunostaining of monoclonal antibocly(PC-l0) and image analysis system,The results showed that the proliferating index(PI)of PCNA pos-itive cells was closely related to AgNORs count and AgNORs'area measured. Both were correlated with the histologicaI type,and the TNM status,decreased with the increasing degree of histological differen-tiation,Survival analysis showed that the PI and the results of AgNORs image analysis in surgical pa-tients who survived over 5 years were significantly lower than those who survived less than 3 years。The results suggest that immunostaining of PCNA and AgNORs image analysis are useful in judging the ma-lignant degree and the prognosis of primary lung cancer。

关 键 词：肺肿瘤 预后 核仁组成区 增殖细胞核抗原 

分 类 号：R734.207[医药卫生—肿瘤] R730.49[医药卫生—临床医学]