桥粒斑蛋白II在肿瘤组织中的表达  

Expression of desmoplakin II in tumors

作　　者：杨举伦[1] 罗学林[1] 蔡琳[1] 邹红[1] 明洪 

机构地区：[1]解放军成都军区昆明总医院病理科,解放军成都军区昆明总医院老年病科,昆明医学附属第一医院中心实验室

出　　处：《中华病理学杂志》1995年第3期170-172,共3页Chinese Journal of Pathology

基　　金：云南省科委应用基础研究基金

摘　　要：桥粒斑蛋白ＩＩ（ＤＰＩＩ）是一种新的上皮和上皮性肿瘤标记物。我们应用针对石蜡切片的抗ＤＰＩＩ单抗和抗细胞角蛋白（ＣＫ）单抗对２ｌ７例肿瘤作了免疫组织化学ＡＢＣ法染色的研究，结果：在１６３例上皮性肿瘤和双向分化的肿瘤中，１４７例表达ＤＰＩＩ，占９０．７％，９２例表达ＣＫ，占５６．４％。ＣＫ阳性染色全部位于细胞浆内，ＤＰＩＩ阳性染色则同时分布在细胞交界面上和细胞浆中。其他５４例不含桥粒的肿瘤均无ＤＰＩＩ和ＣＫ表达。结果表明，ＤＰＩＩ单抗是有用的含桥粒肿瘤的免疫组化探针。因ＤＰＩＩ不存在于一切含桥粒肿瘤，故在鉴别上皮性肿瘤时，ＤＰＩＩ单抗尚需与其他上皮标记物的抗体合并使用。esmoplakin II(DPII )is a new marker for identification of epithelium and epithelial tumors。Ex-pression of DPII in 2l7 tumors cases was studied immunohistochemically by ABC methos using an anti-DPII monoclonal antibody which reacts with tissue on formalin-fixed,paraffin-embedded sections。A monoclonal antithey against cytokeratin(CK) was employed to compare with the antibody. Immuno-histochemical staining showed that l47 of the l63 epithelial tumors and bi-directional differentiation tu-mors expressed DPII(90.7%);92 of the l63 tumors expressed CK(56.4%);54 tumors lacking desmosome expressed neit her DP II nor CK, AII CK posit ive staining was located in cytoplasm while most DPII positive dotty staining was located at the cell-cell borders some DPII staining was present in cytoplasm,These results demonstrate that the anti-DPII monoclonal antibody is a useful immunohis-tochemical probe for identification of desmosome-containing tumors。 Antibodies against other epithelial markers such as keratin should be employed together with anti-DPII monoclonal antibody when differen-tiation is made between epithelial and non-epithelial tumors.

关 键 词：肿瘤 诊断 免疫组织化学 桥粒斑蛋白 单克隆抗体 

分 类 号：R730.43[医药卫生—肿瘤]