染矽尘大鼠早期肺组织Ⅰ、Ⅲ型胶原的表达  

Expressions of collagens Ⅰ,Ⅲ in rat lungs exposed to silica early

作　　者：杜海科[1] 刘彦[2] 王世鑫[1] 常爱华[1] 

机构地区：[1]武警医学院附属医院科研科,天津300162 [2]天津市东丽区卫生防病站职业卫生科,天津300300

出　　处：《武警医学》2005年第7期486-489,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金资助(30070654)

摘　　要：目的探讨染矽尘大鼠早期肺组织中Ⅰ型、Ⅲ型胶原形成的时间规律。方法采用气管暴露法建立矽肺动物模型。取肺组织制作石蜡切片,天狼猩红法染色,偏振光显微镜观察Ⅰ、Ⅲ型胶原的表达,用图像分析系统对Ⅰ、Ⅲ型胶原进行定量分析。结果染矽尘后第3天,Ⅲ型胶原开始增生,染矽尘后第7天,Ⅰ型胶原开始增生,直至染矽尘后第28天。这2种胶原进行性增加。Ⅰ、Ⅲ型胶原阳性面积百分比在染矽尘后均有不同程度的增加。染矽尘后第7天、第14天、第21天、第28天,Ⅰ型胶原阳性面积百分比分别增加27.12(P<0.01),24.73(P<0.01),16.01(P<0.05),52.45(P<0.01)。染矽尘后第3天、第28天,Ⅲ型胶原阳性面积百分比分别增加2.20(P<0.01)、24.13(P<0.01)。结论染矽尘大鼠Ⅰ、Ⅲ型胶原明显增加。Ⅰ、Ⅲ型胶原增生出现的时相有所不同,染矽尘后第3天,Ⅲ型胶原开始出现,染矽尘后第7天,Ⅰ型胶原开始形成,此后,Ⅰ、Ⅲ型胶原呈进行性增多的趋势。天狼猩红-偏振光显微镜法是一种定量检测Ⅰ、Ⅲ型胶原的更加准确的方法。Objective To evaluate the effects of silica on temporal expressions of collagens Ⅰ,Ⅲ in rat lung.Methods Animal models were developed by intratracheal instillation of silica into rat lungs exposed surgically.Paraffin-embedded lung sections were stained with Sirius red,and detected for collagens Ⅰ,Ⅲ by polarization microscopy and Image-Pro Plus Version 4.5 for WindowsTM.Results On 3rd day after silica instillation,green collagen Ⅲ started to appear;7th day after silica instillation,red collagen Ⅰ was formed;and thereafter collagens Ⅰ,Ⅲ were increased progressively.The collagen Ⅰ,Ⅲ positive area percentages were augmented in silica-treated rats.7,14,21,and 28 d after silica instillation,collagen Ⅰ positive area percentages increased 27.12 (P<0.01),24.73 (P<0.01),16.01 (P<0.05),and 52.45 (P<0.01), respectively.3 and 28 days after silica instillation,collagen Ⅲ positive area percentages increased 2.20 (P<0.01) and 24.13 (P<0.01),respectively.Conclusions Collagens Ⅰ,Ⅲ are increased significantly in silica-treated rats. There is a sequence in the course of production of collagens Ⅰ,Ⅲ.On day 3 after silica instillation,green collagen Ⅲ starts to appear;day 7 after silica instillation,red collagen Ⅰ is formed;and thereafter collagens Ⅰ,Ⅲ are increased progressively. Sirius red staining in anjunction with polarization microscopy is an accurate method for assaying collagens Ⅰ,Ⅲ.

关 键 词：Ⅲ型胶原 肺组织 矽尘 大鼠 早期 图像分析系统 Ⅰ型胶原 天狼猩红 时间规律 动物模型 石蜡切片 微镜观察 定量分析 不同程度 胶原增生 定量检测 显微镜法 偏振光 进行性 暴露法 百分比 面积 阳性 比分 出现 

分 类 号：R575.2[医药卫生—消化系统] R563[医药卫生—内科学]