Slit-Robo信号对口腔癌Tb细胞增殖作用的影响  

Effect of Slit-Robo signal on the proliferation of oral cancer cell line Tb

作　　者：马宇光[1] 王丽京[1] 韩冰[1] 

机构地区：[1]兰州大学口腔医院,730000

出　　处：《实用口腔医学杂志》2005年第4期441-444,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(项目批准号:30470858)

摘　　要：目的:探讨SlitRobo信号对人舌鳞状上皮癌细胞系Tb细胞生长与增殖的影响及其作用机制。方法:采用Westernblot免疫印迹法检测Tb细胞中Slit和Robo蛋白的表达;在Robo1受体胞外区的单克隆抗体R5作用下,以MTT比色法与平板克隆形成实验观察Tb细胞生长变化及用免疫组化检测增殖相关蛋白PCNA的表达。结果:Tb细胞表达Slit和Robo蛋白;在R5抗体的作用下,Tb细胞体外生长能力受到抑制,增殖能力明显减弱,PCNA蛋白表达水平下降。结论:SlitRobo信号能够调控Tb细胞的生长增殖能力,对肿瘤细胞内的PCNA有正调控作用,提示该信号可能参与了口腔肿瘤的发生、发展过程。Objective:To study the effect of Slit-Robo signal on the proliferation of human oral squamous cell carcinoma line Tb. Methods:The expression of Slit and Robo protein was measured with Western blot. After the addition of the monoclonal antibodies (mAb) of R5 against Robo1 receptor extracellular domain, the changes of cell proliferation were studied by MTT assay and clone formation assay,the expression of PCNA was measured with immunohistochemical staining. Results:Western blot showed that Slit and Robo proteins were expressed in Tb cells. The proliferation rate of Tb cells decreased and the expression of PCNA decreased after the addition of R5 mAb. Conclusion:Slit-Robo signal may regulate the proliferation of Tb cells by up-regulation of PCNA.

关 键 词：Slit—Robo信号 Tb细胞 R5抗体 增殖 

分 类 号：R739.8[医药卫生—肿瘤]