VX_2种植瘤^(99m)Tc-HL91 SPECT和^(18)F-FDG PET/CT显像的对比研究  

Comparative study of ^(99m) Tc-HL91 SPECT and ^(18)F-FDG PET/CT in experimental VX_2 rabbit tumors

作　　者：任瑞美[1] 袁明智[2] 于金明[1] 杨国仁[3] 左文述[4] 邢力刚[1] 赵伟[1] 孔莉[1] 付正[3] 李国梁[3] 

机构地区：[1]山东省肿瘤防治研究院放疗科,山东济南250117 [2]临沂市人民医院放射科,山东临沂271000 [3]山东省肿瘤防治研究院CTPET研究中心,山东济南250117 [4]山东省肿瘤防治研究院外科,山东济南250117

出　　处：《肿瘤防治杂志》2005年第12期881-884,共4页China Journal of Cancer Prevention and Treatment

基　　金：国家自然基金资助(C030330302)

摘　　要：目的:用荷瘤兔模型研究乏氧显像剂(4,9diaza3,3,10,10tetramethyldodecan2,11dionedioxime,99mTcHL91)和18F氟代脱氧葡萄糖(18Ffluorodeoxyglucose,18FFDG)在肿瘤乏氧诊断中的应用价值。方法:1)体外试验,分别观察在常氧及乏氧状态下对VX2细胞摄取99mTcHL91和18FFDG的影响。2)体内试验,分别于耳缘静脉注入99mTcHL91后3h和18FFDG后1h,采集荷瘤兔的图象,随后,处死荷瘤兔取脑、肺、心、肝、脾、肾、坏死和非坏死肿瘤等标本称质量,测定放射性计数。肿瘤组织切片行HE染色,观察其特点。结果:1)乏氧与常氧状态下比较能引起VX2细胞对99mTcHL91和18FFDG的摄取并随时间延长而增加,P<0.05。2)荷瘤兔肿瘤显像清晰,肿瘤未坏死部位的放射性分别是坏死部位的4倍(99mTcHL91)和3.5倍(18FFDG)。HE染色显示瘤内有大量的坏死组织。结论:乏氧能引起VX2细胞对99mTcHL91和18FFDG的摄取增加;VX2荷瘤兔的瘤体在99mTcHL91和18FFDG显像上均为清晰的阳性病灶,坏死肿瘤组织的放射性低于非坏死肿瘤组织;两者均不能将乏氧的部位明确地显示出来。OBJECTIVE: To elucidate the application of 99m Tc-HL91 and 18 F-FDG in hypoxic diagnosis by the model of tumor bearing rabbit. METHODS: The uptake of 99m Tc-HL91 and 18 F-FDG under hypoxic and normoxic condition were compared respectively. In vivo, 99m Tc-HL91 and 18 F-FDG was injected intravenously, images were acquired 3h and 1h respectively, then the rabbits were killed. The specimen of muscle, brain, heart, liver, spleen, kidney, lung, necrotic tumor and non-necrotic tumor were weighted and radioactivity was counted by γ-counting well. The character of tumor was observed by HE stained. RESULTS: The uptakes of 99m Tc-HL91 and 18 F-FDG in hypoxic cells were higher than nomorxia cells. The uptakes increased with time prolonged, P<0.05. The tumors were found clearly in 99m Tc-HL91 SPECT and 18 F-FDG PET/CT images. But the radioactivity of venter was higher than tumor. The radioactivity of non-necrotic tumor was 4 times or 3.5 times of that of necrotic tumor using 99m Tc-HL91 or 18 F-FDG respectively. HE stained indicated that there was a lot of necrotic tissue in tumor. CONCLUSIONS: The uptake of 99m Tc-HL91 and 18 F-FDG were higher in hypoxic VX_2 cells than normoxic one; 99m Tc-HL91SPECT and 18 F-FDG PET/CT could identify regional tumor in rabbit bearing VX_2 cell. The radioactivity was higher in non-necrotic tumor than necrotic tumor; they could not identify hypoxic regional clearly.

关 键 词：免VX2 显像 ^99mTc—HL91 ^18F-FDG 

分 类 号：R730.4[医药卫生—肿瘤]