喉癌引流淋巴结淋巴细胞在喉癌动物模型体内的分布  

In Vivo Tissue Distribution of Laryngeal Cancer Drained Lymph Node Lymphcytes in Tumor-Baring Mice

作　　者：吴园丁[1] 郭敏[1] 

机构地区：[1]北京医科大学第一临床医学院耳鼻咽喉科

出　　处：《中华耳鼻咽喉科杂志》1995年第4期197-199,共3页Chinese Journal of Otorhinolaryngology

摘　　要：用氚标胸腺嘧啶核苷（￣３Ｈ－ＴｄＲ）标记人喉癌引流淋巴结淋巴细胞，转输入皮下接种人喉癌瘤系ＰＨＣ＿３的裸鼠体内，检测各脏器及肿瘤内的放射性强度而计算出标记细胞的分布百分率。结果发现转输后第１天３０．７％的标记细胞位于肝脏，肺、脾及肾也有少量分布。以后分布量逐周减少，到第４周末肝脏中仍有４．３％分布。而肿瘤中则始终只有极少量标记细胞存在。结果提示人喉癌引流淋巴结淋巴细胞对人喉癌没有专嗜性，不能聚集到肿瘤局部。活化淋巴细胞对肿瘤的杀伤机制除了直接接触杀伤外，还可能有更复杂的间接杀伤机制存在。The distribution of￣3H-TdR labled human laryngeal cancer drained lymph node lym-phocytes(LNL)to human laryngeal squamous cell carcinoma were traced in PHC_3 bearing nude mice,and the radioactivity in tissues was detceted.30.7% labeled cells were localized in live in first day after transfer,and a few in lung,spleen and kidney.Labeled cells were reduced weekly and by the end of the fourth week there were still 4.3%in live,but very few were detected in the tumor site.This indicatd that cancer drained LNL had no specificity to tumor, and could not infiltrate into tumor sites.So besides directly attacking tumor cells in vivo possibly,there are some indirect mechanisms to mediate tumor re-gression.

关 键 词：淋巴细胞 喉肿瘤 淋巴结 动物模型 专嗜性 分布 

分 类 号：R739.650.3[医药卫生—肿瘤]