海洋来源白浅灰链霉菌中环二肽类化合物的分离与结构鉴定  

Isolation and Identification of Cyclic Dipeptides from a Marine-DerivedStreptomyces albogriseolus

作　　者：古静燕[1] 刘红兵[1] 崔承彬[1,2] 顾谦群[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,海洋药物与食品研究所 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国海洋大学学报（自然科学版）》2005年第4期618-620,共3页Periodical of Ocean University of China

基　　金：国家高技术研究发展计划项目(2003AA624020;2001A624020);国家杰出青年科学基金(39825126);国家自然科学基金(30300433);山东省科技攻关计划(0121100107);山东省自然科学基金重点(Z2001C01);国家教育部长江学者奖励计划基金资助

摘　　要：为了寻找新的抗肿瘤活性化合物,采用硅胶、SephadexLH20柱色谱和高效液相色谱,从白浅灰链霉菌Streptomycesalbogriseolus(A2002)中分离得到3个环二肽;利用理化及波谱学方法鉴定其结构分别为环(丙氨酸缬氨酸)[cyclo(AlaVal),1],环(丙氨酸脯氨酸)[cyclo(ProAla),2]和环(脯氨酸酪氨酸)[cyclo(ProTyr),3]。In the course of our screening for new anti-tumor compounds from marine-derived microbial strains, it was found that the acetonic extract from the fermentation of Streptomyces albogriseolus A2002, a marine-derived actinomycete strain isolated from a Jiaozhou Bay sediment sample, significantly inhibited the cell cycle of tsFT210 cells at the G2/M phase and induced apoptosis in tsFT210 cells. Thus, the chemical examination was undertaken on the constituents of bioactive fractions obtained by bioassay-guided fractionation from the fermentation of producing strains. Compounds 1-3 were isolated by repeated column chromatography over Sephadex LH-20 and silica gel, followed by the prepared HPLC separation. Their structures were identified as cyclic dipeptides, cyclo-(Ala-Val) (1), cyclo-(Ala-Pro) (2) and cyclo-(Pro-Tyr) (3), respectively, by the spectroscopic method.

关 键 词：链霉菌 结构鉴定 STREPTOMYCES 分离 SEPHADEX 肽类 海洋 高效液相色谱 活性化合物 谱学方法 丙氨酸 脯氨酸 抗肿瘤 柱色谱 环二肽 缬氨酸 酪氨酸 

分 类 号：O623.423[理学—有机化学] TQ465.2[理学—化学]