鼠肾包膜下人卵巢癌移植瘤体积变化及病理学对照研究  

The analysis of tumor growth rate and histopathol ogy of xenografts of nude mice bearing human ovarian carcinoma in immunocompetent mice

作　　者：王建六[1] 钱和年[1] 李文锦[1] 冯捷[1] 

机构地区：[1]北京医科大学附属人民医院妇科肿瘤研究室

出　　处：《中华妇产科杂志》1995年第3期157-160,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：人卵巢癌细胞系（ＳＫＯＶ＿３）经裸鼠传代后，移植于正常免疫功能Ｃ５７ＢＬ／６纯系小鼠肾包膜下，双侧肾脏多位点移植，３２只小鼠共移植瘤块１２９个，分别在移植术后４、６、８、１０、１２和１４天切取移植瘤，测量体积变化并观察病理组织学特征。结果：移植瘤在术后６～８天体积增加较快；１２～１４天增加幅度明显降低（Ｐ＜０．０５）；宿主淋巴细胞浸润，在８～１２天时达高峰，移植术后１４天，移植瘤几乎全部纤维化；光镜下瘤细胞可见率，术后４天为７７．２３％，以后逐渐下降，至１２～１４天，明显降低。提示：双侧肾脏多位点移植法简便易行。经裸鼠传代的人卵巢癌细胞可在正常免疫功能小鼠肾包膜下生存８～１０天。移植瘤体积变化并不能准确反映瘤细胞生长状况，尚需结合组织病理学检查。enografts of nude mice bearing human ovarian carcinoma cell line SKOV_3 were transplanted using subrenal capsule assay (SRCA)in immnunocompetent C57BL/6 mice. Multiple locations were chosen on both sides of the kidneys obtaining a total of 129 tumors.The volumes of the tumors were rneasured and histopathologic examinations were conducted on the 4th, 6th,8th,10th,12th and 14th days after transplantation surgery.The growth rates were noticed to be high on the 6～8th days.Lymphocytic in-filtration reached a peak on the 8～12th day,On the 14th days the leukocytic infiltration and fibrosis al-most surrounded the tumors.Tumor cells that could be found amounted to about 77.25%,65.38%,and 52. 17%respectively on the 4th,6th and 8th days,but significantly decreased on 12～14th days. The above results suggested :(1)multiple SRCA on both sides proved to be practical and economical;(2) the xenografts of nude mice bearing human ovarian carcinoma could be transplanted viably by SRCA for 8～10 days;(3)microscopic measurement of the transplanted tumor volume did not represnt the real. status of the tumor growth,and microscopic evaluation was necessary.

关 键 词：卵巢肿瘤 肾包膜下 病理学 移植病 

分 类 号：R737.310.2[医药卫生—肿瘤]