人卵巢癌细胞雄激素受体测定及性激素对细胞增殖的影响  

Androgen receptors and effects of sex hormones on growth Of a human ovarian carcinoma cell line

作　　者：卢建[1] 张金山[1] 曾燕[1] 牟翰舟 许沈华 

机构地区：[1]第二军医大学病理生理教研室,浙江肿瘤研究所

出　　处：《中华妇产科杂志》1995年第6期360-362,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：以人工合成的雄激素［１７α－甲基￣３Ｈ］－三烯醇酮（￣３Ｈ－Ｒ１８８１）为配体，采用完整细胞受体测定法，测定人卵巢癌ＨＯ－８９１０细胞的雄激素受体（ＡＲ），并观察不同浓度（０．０１～１μｍｏｌ／Ｌ）的雌二醇、孕酮及睾酮对ＨＯ－８９１０细胞增殖的影响。结果：ＨＯ－８９１０细胞具有高亲和力、低容量的雄激素特异结合位点，用Ｓｃａｔｃｈａｒｄ分析法求得其最大结合容量（Ｒｏ）及表观解离常数（Ｋｄ）分别为６．５２ｆｍｏｌ／１０￣６细胞及２．９６ｎｍｏｌ／Ｌ。表明：ＨＯ－８９１０细胞存在ＡＲ。经上述性激素作用后，ＨＯ－８９１０细胞的增殖率没有明显的变化，表明性激素对该细胞的增殖无明显影响。revious study had showed that ovarian carcinoma cell line (HO-8910 cells) contained estrogen re-ceptors(ER)and progesterone receptors(PR). In this experiment,androgen receptors(AR)in these cells were determined by a whole cell binding assay with a synthetic nonmetabolizable androgen-methyl-trienolone(￣3H-R1881).The results showed that there existed high affinity and low capacity, specific binding sites for ￣3H-R1881 in these cells,the apparent maximum binding capacity and dissociation con-stant of these bindings sites revealed by scathchard analysis being 6. 52 fmol/10￣6 cells and 2. 96 nmol/L respectively,indicating the presence of AR in addition to ER and PR in HO-8910 cells. Furthermore,the effects of sex hOrmones,i.e., estrodiol, progesterone and testosterone on the growth of HO-8910 cells were also investigated. The results showed that testosterone at 10 nmol/L slightly increased the number of HO-8910 cells,but the increase was not dose-dependent and not statistically significant. Es-trodiol and progesterone had no growth-stimulatary or inhibitary effects on these cells.

关 键 词：受体 雄激素 性激素 卵巢肿瘤 细胞生长 

分 类 号：R737.310.2[医药卫生—肿瘤]