多指标综合评分法优选西洋参提取工艺  

Applying Grading Methods of Synthesizing Multiple Guidelines to Optimizing Extracting Technology for Radix Panacis Quinquefolii

作　　者：雷晓林[1] 束家有[1] 招翠微 

机构地区：[1]武汉大学药学院,武汉430072 [2]广州固志医药科技开发公司,广州510520

出　　处：《中药材》2005年第7期599-601,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:优选西洋参最佳提取工艺。方法:(1)采用正交试验法,以西洋参中人参皂苷Rg1、Re、Rb1的总转移率及浸膏得率为指标,多指标综合评分,优选西洋参的乙醇提取工艺条件;(2)运用RP-HPLC梯度洗脱法同时测定西洋参中的人参皂苷Rg1、Re、Rb1的含量。结果:西洋参乙醇提取的最佳条件为12倍量70%乙醇回流提取2次,每次提取3h。结论:该工艺可以有效提高西洋参的提取率。Objective: To optimize the best extracting procedure for Radix Panacis Quinquefolii. Methods: (1)The optimum alcohol extracting procedure was selected with the content and extraction efficiency of ginsenoside Rg_1?Re?Rb_1 from Radix Panacis Quinquefolii by orthogonal test design and synthesizing multiple guidelines method. (2)The content of three ginsenoides Rg_1?Re and Rb_1 in Radix Panacis Quinquefolii was determined by gradient elution method in RP-HPLC. Results: The optimum alcohol-extracting procedure was determined as 12 times of 70% alcohol refluxing and extracting 3h for 2 times. Conclusions: The optimum extracting procedure can increase extraction efficiency of Radix Panacis Quinquefolii.

关 键 词：多指标综合评分法 西洋参 优选 人参皂苷Rg1 HPLC梯度洗脱法 最佳提取工艺 提取工艺条件 正交试验法 浸膏得率 同时测定 回流提取 最佳条件 乙醇提取 转移率 提取率 Re Rb 

分 类 号：R283.64[医药卫生—中药学] R282.71[医药卫生—中医学]