口腔癌引流区淋巴结细胞对人舌鳞癌细胞的杀伤机理  

A preliminary study on the mechanism of againsthuman tongue cancer cell lin

作　　者：郭伟[1] 邱蔚六[1] 何荣根[1] 

机构地区：[1]上海第二医科大学口腔医学院颌面外科

出　　处：《中华口腔医学杂志》1995年第3期143-145,共3页Chinese Journal of Stomatology

摘　　要：以人基因重组白细胞介素２（ｒＩｌ－２）激活的人口腔癌引流区淋巴结细胞（ＤＮＬ），具有杀伤人舌鳞癌细胞系Ｔｃａ８１１３细胞的作用。在光镜下观察到，当二者共同培养１２天后，形成似玫瑰花环状形态，靶细胞出现水疱变性。透射电镜下观察到，ＤＮＬ细胞形态呈多样，核远离与靶细胞接触的一侧；其胞浆内出现较多的分泌颗粒和线粒体，且朝向细胞伸出伪足和突起与靶细胞接触的一侧。靶细胞受攻击后表现为“凋零状坏死”（ａｐｏｐｔｏｓｉｓ）。本研究结果提示，ＤＮＬ细胞的杀伤机理之一，可能通过直接接触释放特定物质，使肿瘤细胞“穿孔”，发生通透性改变，最终导致与一般病理状态下细胞死亡有所不同的凋零状坏死。AbstractInverted phase contrast microscope and transmis-sion electron microscope examination reveals that, tu-mor-draining lymphnode lymphocytes (DNL)conjugat-ed with tumor cells very tightly at 12 hours co-culture.The DNL cell nucleus was seen positioned away fromthe target cells, whereas the cell mitochondria granuleswere oriented toward the point of contact with the tar-get cells. Tke injured target cell had a highly condensedcytoplasm and chromatin and formation of cytoplasmicblebs, which were consistent with an apoptotic celldeath rather than with a lytic or necrotic cell death. Themechanism was exocytosis of granules containing a pore-forming protein. Holes formed in tlie target cell’s mem-brane may allow a lethal substance to enter and to in-duce its death via an apoptotic mechanism.

关 键 词：口腔肿瘤 癌 淋巴结构 超微结构 病理 

分 类 号：R739.802[医药卫生—肿瘤]