离体热暴露大鼠心肌细胞内钙及Ca^（2＋）－ATP酶活力的变化  

THE INTRACELLULAR CALCIUM AND Ca2+-ATPase IN RAT CARDIOMYOCYTES AFTER HEAT EXPOSURE IN VITRO

作　　者：杨晓昊[1] 陈西京[1] 程素琦[1] 李文选[1] 

机构地区：[1]天津军事医学科学院卫生学环境医学研究所

出　　处：《中华劳动卫生职业病杂志》1995年第1期7-8,共2页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：观察了离体成年大鼠心室肌肌质网、线粒体Ｃａ２＋－ＡＴＰ酶活性和钙含量及培养乳鼠心肌细胞内游离钙浓度（心肌［Ｃａ２＋］ｉ）在不同温度热暴露４０ｍｉｎ后的变化。结果表明：热暴露后，心肌匀浆、肌质网及线粒体中的Ｃａ２＋－ＡＴＰ酶活力有所下降，心肌肌质网及线粒体中的钙含量亦有降低趋势，心肌［Ｃａ２＋］ｉ显著升高。提示：热暴露后心肌细胞钙超载是导致机体心功能损害的重要原因之一。The activity of C2+-ATPase,calcium content of sarcoplasmic reticulum SR and mitochondria of myocardium in adult rats and intracellular Ca2+in primary culture cardiomyocytes prepared from neonatal rats were observed after exposure to 37 ℃, 39℃, 41℃,and 43℃ for 40 min respectively in vitro.The activity of Ca2+-ATPase was measured by hydrolysis of ATP to release inorgnic phosphorus,the intracellular Ca2+by the fluorescent Ca2+indicator,Fura-2/AM.The results showed that the calcium content and activity of Ca2+-ATPase in SR and mitochondria decreased gradually as exposure tempeature increased, the activity of Ca2+-AT-Pase in SR and mitochondria decreased by 28.8%, 26.9%,respectively at 43℃.The intracellular Ca2+concentration increased by 16.7%,26.5%,and 46.0%respectively at 39℃,41℃,and 43℃ It is suggested that the decrease of activity in Ca2+-ATPase may lead to the rise of intracellular Ca2+which maybe one of the cause of cardiac function disorder or heart failure in heat stress.

关 键 词：热暴露 心肌细胞 细胞内钙 钙离子 大鼠 

分 类 号：R363.123[医药卫生—病理学] R135.3[医药卫生—基础医学]