巨噬细胞炎症蛋白1-α、解整合素样-金属蛋白酶8、12和CD68蛋白在颌骨巨细胞病变及长骨骨巨细胞瘤中的表达  

Expression of macrophage inflammatory protein-1α, a disintegrin-like and metalloproteinase 8 and 12, and CD68 protein in giant cell lesions of jaw and giant cell tumors of long bone

作　　者：孟雪梅[1] 于世凤[1] 陆敏[2] 郑杰[2] 韩志惠[2] 

机构地区：[1]北京大学口腔医学院病理研究室 [2]北京大学医学部病理学系

出　　处：《中华病理学杂志》2005年第7期393-396,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目(30271412)

摘　　要：目的检测巨噬细胞炎症蛋白1-α(MIP-1α)、解整合素样-金属蛋白酶(ADAM)8、12和CD68在颌骨巨细胞病变和长骨骨巨细胞瘤中的表达,探讨其在这两种巨细胞病变中的多核巨细胞发生中的作用。方法采用免疫组织化学SP法检测24例颌骨中心性巨细胞病变,24例长骨骨巨细胞瘤石蜡组织中的MIP-1α、ADAM8、ADAM12和CD68蛋白的表达。结果MIP-1α在24例颌骨中心性巨细胞病变和24例长骨骨巨细胞瘤中的血管、类骨质和新骨形成区中强表达,而在多核巨细胞和圆形单核细胞中为阴性;ADAM8、ADAM12和CD68在颌骨中心性巨细胞肉芽肿和长骨骨巨细胞瘤中几乎所有多核巨细胞和部分圆形单核基质细胞均为阳性。结论颌骨中心性巨细胞病变和长骨骨巨细胞瘤中的多核巨细胞可能由CD68+的圆形单核基质细胞融合而成,这些圆形单核细胞可能是由骨微环境中的趋化因子,募集外周血中循环的单核细胞到病变局部分化而成的具有破骨细胞前体细胞性质的细胞,继而在某些融合蛋白作用下发生融合,成熟为多核破骨样巨细胞。Objective To detect the expression of macrophage inflammatory protein-1α(MIP-1α), a disintegrin-like and metalloproteinase (ADAM) 8 and 12 and CD68 protein in giant cell lesions of jaw and giant cell tumors of long bone, and to study their effects on the histogenesis of giant cells in such lesions. Methods MIP-1α, ADAM8, ADAM12 and CD68 were detected by immunohistochemistry in 24 paraffin-embedded specimens of central giant cell lesions of jaw and giant cell tumors respectively. Results MIP-1α positive signal was located in blood vessels and bone. ADAM8, ADAM12 and CD68 positive signals were located in the cell membrane and cytoplasm of all multinucleated giant cells and some round mononuclear cells in the lesions. In addition, some spindle mononuclear stromal cells were positive for ADAM12 in both lesions. Conclusion Multinucleated giant cells probably originate from CD68-postive round mononuclear cells, which are recruited from monocyte-macrophage system by chemokines, such as MIP-1α, followed by cell fusion mediated by ADAM8 and ADAM12.

关 键 词：长骨骨巨细胞瘤 巨噬细胞炎症蛋白 颌骨巨细胞病变 解整合素样-金属蛋白酶8 CD68 MIP-1α 免疫组织化学SP法 多核巨细胞 单核细胞 单核基质细胞 巨细胞肉芽肿 中心性 细胞发生 石蜡组织 新骨形成 细胞融合 趋化因子 骨微环境 

分 类 号：R738[医药卫生—肿瘤]