恒河猴脑选择性超深低温断血流复苏模型的建立  

Establishment of maca mulatta model of resuscitation after selective cerebral ultra-deep hypothermic blood flow occlusion

作　　者：周杰[1,2] 冯忠堂[1] 高永军[1] 方绍龙[1] 付登礼[1] 江基尧[1] 徐蔚[1] 

机构地区：[1]昆明医学院第二附属医院神经外科,650101 [2]中国中医研究院望京医院外科

出　　处：《中华外科杂志》2005年第13期889-891,共3页Chinese Journal of Surgery

基　　金：云南省自然科学基金重点项目资助(2003C0010Z);教育部科学技术研究重点项目资助(02141);云南省自然科学基金资助项目资助(2002C0052M);上海市科委基础研究重点项目资助(03JC14038)

摘　　要：目的探讨猴脑选择性超深低温断血流复苏的可行性。方法4至10岁健康恒河猴10只,动物随机分为3组:4血管(双侧颈总动脉及椎动脉)阻断冷灌注组4只,2血管(双侧颈总动脉)阻断冷灌注组4只,等温灌注组2只。手术前后行头颅MRI检查,手术中监测生命体征及血流动力学指标变化,术后行神经功能缺失评分。结果在10只实验猴中,2血管阻断冷灌注组4只恒河猴术中、术后血流动力学稳定,灌注60min后安全复苏并长期存活,术后头颅MRI检查未见异常,术后神经功能评分无异常;等温灌注组2只恒河猴及4血管阻断冷灌注组4只恒河猴于灌注60min后均未能安全复苏,全部死亡。结论阻断双侧颈总动脉行猴脑选择性超深低温60min后可安全复苏。Objective To study the feasibility of resuscitation after selective cerebral ultra-deep hypothermia and blood flow occlusion. Methods Ten 4-10 year-old maca mulattas were divided into 3 groups: four-vessel occlusion group, two-vessel occlusion group and identical temperature perfusion group. MRI were examinated before and after operation, the vital signs and the hemodynamical parameters were observed during the experiment, neurological deficient evaluation was performed after operation. Results In all of the ten monkeys, the hemodynamical parameters of two-vessel occulation were steady during the operation, and all of them lived after filling 60 minutes. MRI were normal after operation, and the function of neurological deficient scale was normal.The others of identical temperature perfusion group and four-vessel occlusion group were not resuscitation after filling 60 minutes and died. Conclusion Monkey could resuscitate from selective cerebral ultra-deep hypothermia and blood flow occlusion of bilateral common carotid artery in 60 minutes.

关 键 词：超深低温 断血流 复苏模型 猴脑 头颅MRI检查 神经功能缺失评分 血流动力学 颈总动脉 神经功能评分 血管阻断 手术中监测 恒河猴 冷灌注 手术前后 指标变化 生命体征 长期存活 温灌注 术后 椎动脉 双侧 安全 异常 

分 类 号：R-332[医药卫生]