Thy-1肿瘤标记物在肺癌组织中的异常表达及其对肺癌患者预后的影响  

Abnormal expression of Thy-1 as a novel tumor marker in lung cancer and its prognostic significance

作　　者：陈晋峰[1] 张力建[1] 赵爱莲[2] 王歈[3] 吴楠[1] 熊宏超[1] 梁震[1] 李吉友[2] 黄信孚[1] 杨跃[1] 

机构地区：[1]北京大学临床肿瘤学院北京肿瘤医院外科,100036 [2]北京大学临床肿瘤学院北京肿瘤医院病理科,100036 [3]北京大学临床肿瘤学院北京肿瘤医院生物诊断与治疗中心,100036

出　　处：《中华医学杂志》2005年第27期1921-1925,共5页National Medical Journal of China

基　　金：北京卫生局科研基金资助项目(2003049)

摘　　要：目的通过免疫组织化学染色的方法了解Thy1在肺癌组织中的表达状况,分析其对于非小细胞肺癌患者预后的影响,并了解Thy1与p53表达之间的相关性,为Thy1在肺癌中表达机理的进一步研究提供思路。方法以组织芯片的形式,采用免疫组化信号催化放大法,对175例包括不同病理类型的肺组织标本中Thy1的表达情况进行检测,并对其中的91例非小细胞肺癌进行Thy1和p53的联合检测。分析这两种蛋白表达之间的相关性,结合临床病理资料及随访结果分析Thy1与患者生存预后的关系。结果Thy1抗体特异性地在不同病理类型肺癌细胞的胞核中有强阳性染色,89例(56.7%)为Thy1细胞核染色阳性。91例非小细胞肺癌患者预后的单因素和多因素分析显示,TNM分期、淋巴结转移和Thy1在细胞核中高表达是影响患者预后的独立因素。分层研究提示,无淋巴结转移组中,Thy1细胞核阴性患者的生存时间明显长于阳性患者(分别为46.42个月和38.56个月,P=0.0207)。Thy1和p53两者表达之间存在显著的相关性(P<0.001)。结论Thy1在肺癌细胞核内特异性高表达是非小细胞肺癌患者预后的影响因素之一。Thy1在细胞核内的异常高表达可以作为肺癌病理诊断的标记物。Objective The purpose of this study was to investigate the expression of Thy-1 immunohistochemically in different lung tumors and its prognostic significance in non-small cell lung cancer (NSCLC) cases. We also evaluate relationship between Thy-1 and p53 expression status so as to find any clue about the mechanism. Methods In this study, we used anti-Thy-1/CD90 antibody to detect the expression pattern of Thy-1 in different lung tumor sections, which were embedded in paraffin blocks. The expressions of Thy-1 in 175 lung tissue cases, including different pathological types, were analyzed as tissue array form. We also detect expression status in 91 NSCLC among these cases and analyze the relationship between Thy-1 and p53. The relationship between Thy-1 expression and patients' survival was studied.Results We first found that anti-Thy-1 antibody can strongly stain a nuclear molecule in different type of lung cancer cells. Among lung cancer cases, 89 (56.7%) cases showed strong nuclear staining for Thy-1 specially. In univariate and multivariate analysis for 91 NSCLC patients we found TNM staging, lymph node status and Thy-1 overexpression in nuclei were independent factors to affect the prognosis of NSCLC patients. In lymph node nonmetastasis subgroup cases, Thy-1 negative patients had significant longer survival than Thy-1 positive cases (mean survival: 46.42 mons vs 38.56 mons, P=0.0207). There was a significant association between Thy-1 and p53 expression (P<0.0001). Conclusion There is a significant overexpressed Thy-1 located in lung cancer cell nucleus as compared to the normal tissue or benign tumor cells of lung, and it is one of the factors effected on the prognosis of NSCLC patients. This finding suggests that Thy-1 maybe a novel latent malignant marker in the lung cancer pathology. The association between Thy-1 and p53 expression in nucleus suggests that p53 protein and Thy-1 may have some interaction.

关 键 词：THY-1 肺癌患者 肺癌组织 肿瘤标记物 预后 异常 表达及 免疫组织化学染色 非小细胞肺癌 临床病理资料 病理类型 细胞核内 P53表达 抗体特异性 多因素分析 TNM分期 淋巴结转移 相关性 高表达 表达状况 组织芯片 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]