局灶性脑缺血海马神经元淀粉样β蛋白及其蛋白前体的表达  

Expression of amyloid beta protein and amyloid precursor protein after focal brain ischemia in human

作　　者：栾天竹[1] 戚基萍[2] 王德生[3] 陈艳昕[2] 

机构地区：[1]哈尔滨医科大学第一临床医院心内科,150001 [2]哈尔滨医科大学第一临床医院病理科,150001 [3]哈尔滨医科大学第一临床医院神经科,150001

出　　处：《中华医学杂志》2005年第25期1770-1772,共3页National Medical Journal of China

基　　金：国家自然科学基金资助项目(30270480);黑龙江省自然科学基金资助项目(D0203)

摘　　要：目的观察人脑缺血后淀粉样β蛋白140(Aβ140)与其淀粉样β蛋白前体(βAPP)在海马CA1区神经元的表达。方法收集43例因脑缺血而梗死的尸检标本,按缺血时间(发病至死亡时间)分为缺血2～6h、7～24h、25～48h、49～72h、73～96h、97～144h组和145～168h组。选取因其他疾病死亡(非脑缺血)尸检标本2例为对照组。采用HE染色方法观察神经细胞损伤情况;免疫组织化学染色检测Aβ140与βAPP在海马CA1区神经元的表达,在镜下对免疫组织化学染色阳性细胞计数,实验结果应用SPSS11.5统计软件进行分析。结果与对照组(2.88个±0.18个/高倍视野)相比,缺血2～6h组Aβ140表达增加(22.44个±2.79个/高倍视野),在73～96h达高峰(36.30个±2.67个/高倍视野),以后有所回落,但仍高于对照组。缺血7～24h组βAPP阳性细胞数量(28.11个±2.03个/高倍视野)显著低于2～6h组(33.30个±0.42个/高倍视野),缺血24h后阳性细胞数量增多,73～96h组达高峰(32.32个±1.36个/高倍视野),96h以后下降,但仍高于对照组(25.90个±1.55个/高倍视野)。24h后βAPP的增加与Aβ140的增加呈显著正相关。结论人脑缺血后Aβ表达增加,并与βAPP协同加重脑缺血性损伤。Objective To observe the expression of amyloid beta precursor protein and amyloid beta protein in neurons of hippocampal CA1 region after brain ischemia in human. Methods Neuronal damage was examined by using HE staining, and expression of APP and Aβ(1-40) was determined by immunohistochemistry in the hippocampal CA1 region of the brain specimens of 43 patients who died 2 h～(6 h),7 h～24 h,25 h～48 h,49 h～72 h,73 h～96 h,97 h～144 h,or 145 h～168 h after cerebral ischemia and in 2 specimens of patients who died of other diseases as control group. Results The expression of Aβ(1-40) was 25.07±2.79 in the specimens 2h～6h after cerebral ischemia, peaked 73 h～96 h after cerebral ischemia(33.22±2.67),then decreased till 145h～168h after cerebral ischemia, however, all higher than that in the control group (2.88±0.18, all P<0.05). The expression of β-APP was 33.30±0.42 2 h～(6 h) after cerebral ischemia, was 28.11±2.03 7 h～24 h after cerebral ischemia, increased to peak value (32.32±1.36) 73h～96h after cerebral ischemia, and then decreased to 28.48±2.01, all higher than that of the control group(25.90±1.55)with significant differences between those 2 h^6 h and 73 h^96 h after ischemia and that of the control group (both P<0.01). The increase of β-APP was positively correlated with the expression of Aβ(1-40) 24 h after ischemia. Conclusion The expression of β-APP and that of Aβ(1-40) are up-regulated after cerebral ischemia, thus aggravating cerebral ischemia.

关 键 词：局灶性脑缺血 海马神经元 AΒ1-40 Β-APP 淀粉样Β蛋白前体 免疫组织化学染色 海马CA1区 白及 HE染色方法 脑缺血性损伤 尸检标本 细胞数量 24h 对照组 死亡时间 缺血时间 疾病死亡 损伤情况 神经细胞 染色检测 细胞计数 

分 类 号：R743.3[医药卫生—神经病学与精神病学]