牙体硬组织表面电位影响骨改建的实验研究  

Effects of surface potentials of tooth hard tissue on bone remodeling in rabbit tibiae

作　　者：张加理[1] 郑幼洋[1] 胡济安[2] 陈学鹏[1] 

机构地区：[1]浙江大学医学院附属第二医院口腔科,杭州310009 [2]浙江大学医学院口腔系

出　　处：《中华口腔医学杂志》2005年第4期331-334,共4页Chinese Journal of Stomatology

基　　金：浙江省卫生厅科学研究基金资助项目(2004A101)

摘　　要：目的研究牙体硬组织表面电位与骨生长改建的关系。方法将人牙釉质试件、牙釉质-牙本质试件、牙本质-牙骨质试件和多孔羟基磷灰石试件各25个植入25只家兔胫骨中,通过组织病理学、酶组织化学染色和四环素荧光标记方法,在术后不同时间段观察、评价各试件正负极侧的家兔胫骨骨组织改建情况。结果光镜观察各时间段牙体试件的负极侧成骨较正极侧活跃,破骨细胞数量计数正极侧较负极侧多(P<0.01)。四环素荧光标记显示负极侧荧光面积多于正极侧(P<0.05)。结论牙体硬组织表面电位可影响骨改建:荷正电的牙釉质面促进骨的吸收破坏,荷负电的牙骨质面促进成骨。牙齿过萌与无牙颌牙槽骨萎缩可能与此有关。Objective To investigate the influence of surface potentials of tooth hard tissue on bone remodeling. Methods After insured the surface potentials of human extracted teeth with electrochemical methods, teeth sections and artificial hydroxyapatite were implanted into 25 rabbits′ tibiae. The rabbits were sacrificed at 1, 2, 4, 6 and 8 weeks after implantation, respectively. The bone regeneration was compared between opposite two sides (cathode side and anode side) of tooth sections using hematoxylin-eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) activity detecting and tetracycline tracing method.Results Resorption lacunae was seen in the tibiae facing to the enamel anode and new bone density in the implant bed near the cathode of tooth samples was much higher than that near the anode, while the number of TRAP positive cells near the cathode was smaller than that near the anode ( P <0.01). The fluorescent area of tetracycline tracing near the cathode was larger than that near the anode ( P <0.05). Conclusions The cathode of tooth hard tissue (cementum) could improve or trigger new bone formation, while the other side, anode (enamel), could improve the bone resorption. This study suggests that tooth hard tissue′s electrochemical characteristic might affect the remodeling of alveolar bone, and tooth supraeruption and the alveolar bone loss after tooth extraction might result from the redundant or lack of root electrochemical stimulation to bone.

关 键 词：牙体硬组织 表面电位 骨改建 实验研究 多孔羟基磷灰石 酶组织化学染色 四环素荧光标记 组织病理学 牙槽骨萎缩 生长改建 人牙釉质 标记方法 组织改建 光镜观察 细胞数量 牙本质 牙骨质 时间段 无牙颌 胫骨 家兔 成骨 

分 类 号：R78[医药卫生—口腔医学]