高脂饮食性脂肪性肝炎大鼠肝脏线粒体损伤和ATP储备改变  

Hepatocyte Mitochondrial Injury and the Change of Adenosine Triphosphate Storage in Rat Nonalcoholic Steatohepatitis

作　　者：丁晓东[1] 范建高[2] 徐正婕[2] 郑晓英[2] 田丽艳[2] 王国良[2] 

机构地区：[1]上海第二医科大学附属新华医院消化内科,上海市200092 [2]上海市第一人民医院消化内科,上海市200085

出　　处：《世界华人消化杂志》2005年第13期1524-1528,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨高脂饮食大鼠脂肪性肝炎模型肝脏线粒体损伤和三磷酸腺苷(ATP)储备的改变. 方法:SD大鼠随机分为模型组(n=48)和对照组(n=24), 分别给予高脂饲料和标准饲料喂养,分批于实验8、12、16和24 wk处死.常规检测血清生化和肝匀浆ATP 含量,光镜和电镜观察肝脏组织学改变. 结果:与对照组相比,模型组肝脏8 wk时肝脂肪变明显,表现为单纯性脂肪肝,12-24 wk进展为脂肪性肝炎;模型组肝脏ATP含量呈减低趋势,16-24 wk时较对照组减低约1/3(P<0.05);电镜下8wk模型组大鼠肝细胞线粒体肿胀、增大,部分内膜嵴粒脱落,16 wk 和24 wk大鼠线粒体内有类圆形结晶样物质沉积. 结论:高脂饮食大鼠脂肪性肝炎模型的肝细胞存在线粒体损伤和ATP储备的减低.AIM: To investigate the mitochondrial injury and change in adenosine triphosphate (ATP) storage in a rat model of nonalcoholic steatohepatitis (NASH) induced by fat-rich diet. METHODS: Seventy-two male SD rats were randomly divided into two groups. The control group (n = 24) was fed with normal diet; the model group (n = 48) was fed with fat-rich diet, which is consisted of 10% lard oil + 2% cholesterol. 6 or 12 rats were sacrificed at the 8th, 12th, 16th and 24th wk of the experiment. Serum biochemical parameters were measured by a commercial laboratory. The hepatic histologic change was evaluated by light microscopy and transmission electron microscopy. The ATP content was measured by luciferin-luciferase method. RESULTS: Simple fatty liver was observed in the model group at the 8th wk. From the 12th wk to 24th wk, the liver gradually progressed to steatohepatitis. The ATP content of the liver was reduced in the model group compared with normal group at the 16th wk [(2.97±0.48)×10-8μmol/g vs (2.25±0.55)×10-8 μmol/g, P= 0.0 361,t=2.354] and the 24th wk [(2.97±0.48)×10-8 μmol/g vs(1.99±0.66)×10-8 μmol/g, P = 0.0 053,t= 3.439]. Markedly abnormal morphology was observed in the hepatocyte mitochondria in model group, including swelling, rounding, loss of cristae, and stacks of intramitochondrial paracrystalline inclusion bodies. CONCLUSION: The hepatocytic mitochondria in NASH were injured and the storage of ATP was reduced, which may contribute to the pathogenesis of NASH.

关 键 词：线粒体损伤 脂肪性肝炎 储备 大鼠肝脏 三磷酸腺苷(ATP) 饮食性 肝脏组织学改变 ATP含量 单纯性脂肪肝 肝炎模型 高脂饮食 线粒体肿胀 大鼠肝细胞 对照组 SD大鼠 高脂饲料 血清生化 常规检测 电镜观察 肝脂肪变 线粒体内 

分 类 号：R575.1[医药卫生—消化系统]