IL-12和IL-6与实验性急性坏死性胰腺炎严重程度的关系  

Correlation of interleukin-12 and interleukin-6 with severity of experimental acute necrotizing pancreatitis

作　　者：赖铭裕[1] 梁志海[2] 唐国都[3] 孙学成[2] 邓德海[3] 

机构地区：[1]广西医科大学第一附属医院老年消化内科,广西壮族自治区南宁市530021 [2]广西医科大学,广西壮族自治区南宁市530021 [3]广西医科大学第一附属医院消化内科,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2005年第13期1550-1553,共4页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目(桂科自);No.0447054~~

摘　　要：目的:探讨血清白介素12(IL-12)浓度在急性坏死性胰腺炎(ANP)中的变化以及他与胰腺病变严重程度的关系. 方法:SD大鼠92只随机分为3组,胰腺炎组(A组, n=36)和干预组(Ⅰ组,n=32)大鼠都分3次60 g/L左旋精氨酸(L-arginine)ip建立ANP大鼠模型;Ⅰ组在诱导胰腺炎后2,5,8 h分3次ip IL-10各10 000 U.正常对照组(C组,n=24)接受同剂量的生理盐水.于4, 12,24,36 h分批处死大鼠,光镜下观察胰腺组织并进行病理评分,用酶底物法检测血清淀粉酶,用ELISA法检测IL-12和IL-6浓度. 结果:A组胰腺病理评分、血清淀粉酶、IL-12及IL-6 的水平显著高于对照组(24 h,血清淀粉酶:3 264.89±1 627.18 vs 364.61±64.24,P<0.01;IL-12:104.68±23.93 vs 56.72±22.67 pg/L,P<0.01:IL-6:132.95±26.64 vs 81.90±9.93 pg/L,P<0.01),Ⅰ组可使各观察指标较A组明显下降(24 h,病理评分:4.75±1.75 vs 7.89±1.17,P<0.01;血清淀粉酶:1481.13±336.48 vs 3 264.89±1 627.18,P<0.01:IL-12:81.31±17.23 vs 1 04.68±23.93 pg/L,P<0.05;IL-6:96.80±1 8.28 vs 1 32.95±26.64 pg/L,P<0.01);IL-12与病理评分(r=0.603, P<0.001)及血清淀粉酶水平(r=0.323,P<0.05)存在正相关,但是与IL-6无显著相关性(P>0.05). 结论:大剂量L-arginine ip诱导的ANP大鼠中,血清IL-12及IL-6显著上升,应用IL-10可减轻胰腺病变程度,IL-12浓度与胰腺病变正相关.AIM: To investigate the change of serum interleukin-12 (IL-12) and interleukin-6 (IL-6) concentrations and the correlation with the severity of pancreas damages in experimental acute necrotizing pancreatitis (ANP). METHODS: Ninety-two Spraque-Dawley rats were randomly divided into ANP (group A, n = 36), IL-10-interfering (group I, n = 32) and control group (group C, n - 24). Rats in group A and I were injected intraperitoneally with 6% L-Arginine (3×1.0 mg/g) three times at an interval of 1 hour to induce ANP and those in group C received saline intraperitoneal injection only. Rats in Group I were treated with recombinment human IL-10 at 2th 5th and 8th hour (10 000 U for each time) after the L-arginine injection. Rats were killed at 4th, 12th, 24th and 36th hour after the last L-arginine injection. The pathological characteristics of the pancreas observed under light microscope. Serum amylase, IL-12 and IL-6 concentrations were detected by substrate-enzyme technique and ELISA respectively. RESULTS: The pathological scores of pancreas, the concentrations of serum amylase, IL-12 and IL-6 in rats of group A were markedly higher than group C (24 h, serum amylase: 3 264.89±1 627.18 vs 364.61±64.24, P<0.01; IL-12:104.68±23.93 vs 56.72±22.67 pg/L, P<0.01; IL-6: 132.95±26.64 vs 81.90±9.93 pg/L, P<0.01). After administration of IL-10, the pathological scores of pancreas, the concentrations of serum amylase, IL-12 and IL-6 in were significantly decreased in group I as compared with those in group A (24 h, pathological scores: 4.75±1.75 vs 7.89±1.17, P<0.01; serum amylase: 1 481.13±336.48 vs 3 264.89±1 627.18, P<0.01; IL-12: 81.31±17.23 vs 104.68±23.93 pg/L, P<0.05; IL-6: 96.80±18.28 vs 132.95±26.64 pg/L, P<0.01). The concentration of IL-12 was positively correlated with pathological scores of pancreas (r= 0.603, P<0.001) and serum amylase concentration (r= 0.323, P<0.05), but had no marked relations with IL-6 concentration (P>0.05). CONCLUSION: The serum concentrations of IL-12 and IL-6 are markedly elevate

关 键 词：急性坏死性胰腺炎 IL-12 IL-6 实验性 血清淀粉酶 IL-10 病理评分 病变严重程度 ELlSA法 左旋精氨酸 正常对照组 SD大鼠 大鼠模型 生理盐水 胰腺组织 镜下观察 观察指标 0.05 病变程度 胰腺病变 ANP 正相关 浓度 白介素 

分 类 号：R576[医药卫生—消化系统]