可溶性肿瘤抗原和CD3单克隆抗体共同诱导的杀瘤细胞——T-AK细胞  

SOLUBLE TUMOR ANTIGENS AND ANTI-CD3' McAb COSTIMULATED TUMOR KILLER CELLS——T-AK CELLS

作　　者：陈毓仙[1] 石卫[1] 万云霞[1] 陈金栋[1] 李艳芬[1] 翟红[1] 

机构地区：[1]中国医学科学院肿瘤研究所,北京100021

出　　处：《中华微生物学和免疫学杂志》1995年第5期293-296,共4页Chinese Journal of Microbiology and Immunology

摘　　要：用可溶性肿瘤抗原和CD3单克隆抗体共同刺激外周血单个核细胞(PBMC)产生CD8^+T细胞为主的杀瘤细胞,称其为T-AK细胞。与LAK细胞和CD3-AK细胞比较,T-AK细胞扩增快、低依赖IL-2,培养14天细胞数扩增24倍,比CD3-AK细胞高8倍,比LAK细胞高15倍,并能持续生长21天。T-AK细胞中含CD3^+86％、CD4^+20％、CD8^+95％、CD16^+40％、CD25^+53％,它们是CD8^+T细胞为主的异质性细胞群。T-AK细胞对刺激它的STA来源的靶细胞高亲和性杀伤,杀伤率>90％,对NK敏感和不敏感细胞也有杀伤,表现非MHC限制杀伤。The study presented that soluble tumor antigens (STA ) and anti-CD3' McAb costimulated peripheral blood mononuclear cells twgen rate tumor killer cells which were predominantly CD8+ T cells,called T-AK cells. Prolifera-tion of T-AK cells was rapid in vitro and low IL-2-dependcnt in comparison with that of LAK cells and CD3 AK cells. T-AK cells expanded 24-flod in 14 days' culture,8-fold higher than CD3-AK cells and 15-fold higher than LAK cells, and even successively grew till 21st day. T-AK cell population contained CD3+86% ,CD4+20% ,CD8 + 95%,CD16+ 40% ,CD25+53% ,and they were heterogenic cell population consisting of mainly CD8+ T cells. T-AK cells killed the related target cells whose STA costimulated them with high affinity,and also killed NK-sensitive and NK-resistant target cells. These results suggest that non-MHC-restricted CD8' cytotoxic T cells could be induced by STA and anti-CD3' McAb costimulation.

关 键 词：可溶性 肿瘤抗原 单克隆抗体 T-AK细胞 

分 类 号：R730.3[医药卫生—肿瘤]