CEA单抗轻重链可变区基因的克隆和序列分析  

CLONING AND SEQUENCE ANALYSES OF THE VL AND V_H GENES FROM ANTI-CEA C50 HYBRIDOMA CELL LINE

作　　者：章美云[1] 孔健[1] 杨宁[1] 黄华梁[2] 

机构地区：[1]卫生部北京生物制品研究所,100024 [2]中国科学院遗传研究所

出　　处：《中华微生物学和免疫学杂志》1995年第6期437-440,共4页Chinese Journal of Microbiology and Immunology

摘　　要：从分泌具有高亲和力和特异性的小鼠抗癌胚抗原(CEA)的单抗杂交瘤细胞株C50中提取总RNA,通过RTPCR扩增并克隆了抗体的轻、重链可变区基因。核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。Mouse anti-carcinoembryonic antigen (CEA) monoclonal antibody C50 (C50 McAb) displayed a high affinity and specificity for CEA. The variable light and heavy chain region (V1. and VH) genes from the C50 McAb hybridoma have been cloned and amplified with RT-PCR. The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.

关 键 词：癌胚抗原 单克隆抗体 可变区基因 克隆 

分 类 号：R730.3[医药卫生—肿瘤]