利用15N－尿素检测幽门螺旋菌感染  

DETECTION OF HELICOBACTER PYLORI IN HUMAN USING 15N-UREA METHOD

作　　者：刘国龙[1] 吴继琮[1] 张振华[1] 牟杨龙 陈强[1] 吴靖川[1] 杨淑蓉[1] 

机构地区：[1]第一军医大学南方医院,上海第二医科大学新华医院,上海第二医科大学微生物学教研室

出　　处：《中华消化杂志》1995年第1期18-20,共3页Chinese Journal of Digestion

摘　　要：应用稳定性核素１５Ｎ－尿素检测幽门螺旋菌（ＨＰ）感染。当受检者服用１５Ｎ－尿素遇ＨＰ的尿素酶分解后释放氨，氨被吸收入血，部分随尿外排。测定尿氨中１５Ｎ－丰度和排出率，可了解１５Ｎ－尿素在胃内分解情况，从而确定有无ＨＰ感染。本法诊断ＨＰ感染的敏感性为９６％，特异性为９７％，重复稳定性良好，适于ＨＰ感染检测，尤适于监测评价药物消除ＨＰ的疗效。e used stable isotope 15N-urea as a tracer to detect the infection of Helicobacter pylori(HP) in humans. A single dose of 15N-urea was administered orally and then urine wascollected everv 30 min for two hours. Gastric biopsy specinnens were taken for histologicalexamination and bacterial culture. Thirty-six subjects were examined using tracer technique,the 15N excretion rate in urine ammonium was much higher in HP positive subjects than thatin HP negative subjects(P<0.05). According to the results of Gram stain and bacteria1culture, the normal range of 15N excretion rate in urine ammonium is below 3%. In 23 HPpositive persons, 22 were positive for 15N excrection test, and in all the 13 HP negativepersons,15N excreation rate was within normal range. Thus the sensitivity of 15N excretiontest in screening HP infection was 96% and specificity 97%.This is a simple accurate, sensithe and noninvasive test for the detection of HP.

关 键 词：氮15 尿素 幽门螺杆菌 胃炎 消化性溃疡 检测 

分 类 号：R573.03[医药卫生—消化系统]