用逆转录－多聚酶链反应监测急性髓系白血病M_（2b）微量残留白血病  

MONITORING OF MINIMAL RESIDuAL LEuKEMIA INACUTE MYELOID LEUKEMIA M_(2b) BY REVERSETRANSCRIPTION-POLYMERASE CHAIN REACTION

作　　者：王建祥[1] 肖志坚[1] 郝玉书[1] 李建波[1] 刘汉芝[1] 薛艳萍[1] 韩明哲[1] 卞寿庚[1] 晁恒军[1] 

机构地区：[1]中国医学科学院血液学研究所

出　　处：《中华血液学杂志》1995年第7期359-362,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金;天津市自然科学基金

摘　　要：急性髓系白血病（ＡＭＬ）Ｍ＿（２ｂ）常伴有ｔ（８：２１）染色体易位，两个染色体重排导致形成ＡＭＬＩ／ＭＴＧ８融合基因，并持续产生融合基因转录本。作者利用逆转录。多聚酶链反应（ＲＴ－ＰＣＲ）扩增ＡＭＬ１／ＭＴＧ８融合基因转录本进行ＡＭＬ－Ｍ＿（２ｂ）微量残留白血病的监测，在１３例完全缓解的患者中，１２例可检出残留白血病细胞。经随诊这１２例中，２例已复发，１例死于强化治疗骨髓抑制期，９例仍持续完全缓解。cute myeloid leukemia (AML)-M_(2b) is frequently as-sociated with mutual translocation of chromosomes 8and 21 , t (8; 21) (q22; q22). The rearrangement ofthese two chromosomes resulted in the formation ofAMLI/MTG 8 fusion gene and the production of a cor-respondent fusion transcript. The minimal residualleukemia were monitored by arnplification of AML1/MTG8 fusion mRNA using reverse transcription-poly-merase chain reaction (RT-PCR). The fusion mRNAswere detected in 12 of 13 AML-M_(2b) patients in com-plete remission (CR) . Out of the 12 PCR positive pa-tients, two relapsed, one died of myelosuppression inintensification chemotherapy and nine remained in con-tinuous complete remission (CCR). The only PCRnegative patient is also in CCR . Our preliminary re-sults suggested that residual leukemic cells carryingAMLllMTGS fusion gene could be detected by RT-PCR assay in most cases of AML-M_(2b) in CR, andseemed not to herald the impending relapse.

关 键 词：急性 髓系 白血病 微量残留 多聚酶链反应 

分 类 号：R733.710.4[医药卫生—肿瘤]