急性白血病骨髓细胞外基质的改变  

STuDY OF EXTRACELLULAR MATRIX OF BONEMARROW IN ACUTE LEUKEMIA

作　　者：陈琪[1] 周淑芸[1] 王时书[1] 蔡俊杰[1] 张进华[1] 

机构地区：[1]第一军医大学南方医院血液科,第一军医大学病理解剖教研室

出　　处：《中华血液学杂志》1995年第7期363-365,共3页Chinese Journal of Hematology

摘　　要：作者应用组织化学、免疫组织化学染色及自动图象分析仪观测５９例急性白血病（ＡＬ）患者１１８次骨髓活检标本骨髓细胞外基质（ＥＣＭ）成分变化及其与造血细胞增殖的关系。其中男性３８例，女性２１例，中位数年龄３４岁。急性非淋巴细胞白血病（ＡＮＬＬ）４１例，急性淋巴细胞白血病（ＡＬＬ）１８例，初诊未治者（ＰＬ）２７例，难治复发者（ＲＬ）３２例，正常对照１０例。结果显示，ＡＬ与正常人骨髓ＥＣＭ成分有显著性差异（Ｐ＜０．０５）；ＡＬ网硬蛋白增加，纤维连接蛋白（Ｆｎ）、酸性粘多糖减低，中性粘多糖初诊未治者增高，难治复发者减低。ＡＬＬ和ＡＮＬＬ之间未见显著性差异；ＲＬ中难以缓解与复发再治者比较，后者改变更明显，而同复发次数无显著性差异。ＰＬ中追踪观察，６／８例（７５％）未缓解患者及８／１９例（４２．１％）完全缓解后复发患者骨髓基质Ｆｎ、酸性粘多糖、中性粘多糖有不同程度改变。骨髓基质Ｆｎ与造血细胞增殖在正常对照组显示呈正相关，而在ＡＬ呈负相关，ＰＬ与ＲＬ尚存在显著性差异（Ｐ＜０．０５）。提示ＡＬ骨髓造血微环境ＥＣＭ有明显变化，与发病机制及预后关系密切。he study of 118 bone marrow samples from 59 pa-tients with acute leukemia(AL) was performed histo-chemically, immunohistochemically and morphometri-cally using IBAs-2000 automatic video analyser. Tennormal adults were as control. The result showed thatthere was significant difference in component of extracellular matrix (ECM) of bone marrow between ALand control group. In AL patients , reticulin increased ,acid glycosaminoglycans and fibronectin decreased,neutral glycosaminoglycans increased in previously un-treated patients but decreased in refractory patients.The cell proliferation showed negative and positive re-lated to fibronectin contents in AL and control groups ,respectively. It suggested thatthe development of ALwas related to both the malignant colony hemopoiesisand the alteration of microenviroment ECM.

关 键 词：急性 白血病 骨髓细胞外基质 免疫组织化学 

分 类 号：R733.710.2[医药卫生—肿瘤]