小儿急性T淋巴细胞白血病tal－1基因缺失分析  

ANALYSIS OF TAL-1 GENE DELETION IN CHILDHOOD ACUTE T CELL LEUKEMIA

作　　者：潘秋炉[1] 仇一华 郭淑芬[1] 卢健[1] 徐淑芝[1] 沈红强[1] 洪文澜[1] 陈诗书[1] 

机构地区：[1]浙江医科大学附属儿童医院,上海第二医科大学分子生物学实验室

出　　处：《中华血液学杂志》1995年第8期417-418,共2页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：根据ｔａｌ－１基因缺失上下游序列设计一对引物，应用多聚酶链反应（ＰＣＲ）技术对１０株人白血病细胞株和７０例小儿急性白血病骨髓标本进行ｔａｌ－１基因缺失分析。结果ＣＥＭ、ＨＢＳ－２和ＲＰＭＩ－８４０２三株Ｔ细胞白血病（Ｔ－ＡＬＬ）细胞株以及３／１６例Ｔ－ＡＬＬ发现ｔａｌ－１基因缺失，５４例其它类型急性白血病均未见基因缺失，而且具ｔａｌ－１基因缺失的Ｔ－ＡＬＬ细胞均为胸腺发育Ｉ期，免疫表型特征为ＣＤ＿２＋、ＣＤ＿７＋、ＣＤ＿１￣－、ＣＤ＿４￣－和ＣＤ＿８￣－。ＰＣＲ方法敏感性达１０￣（－４）。说明ｔａｌ－１缺失发生于部分Ｔ－ＡＬＬ中，可用于微小残留病的检测。wo primers were designed according to up-anddown stream sequences of the deletion site of tal.1gene. TaL-1 gene deletion was assayed by PCR methodhten human leukemia cell lines and 70 children withacute leukemia.The deletions were detected in three Tcell eukemia cell lines (CEM, HBS-2, RPMI 8402 )and 3 of 16 T-ALL patients, The leukemic cells ofthree patients with tal-1 gene deletion were at stage Iof thymus differentiation with a immunophenotype ofCD_2￣ +,CD_7￣+,CD_1￣-,CD_4￣-,CD_8￣-,The sensitivity of PCR was 10￣(-4). It suggested that tal-1 gene deletionoccured in some of T-ALL patient and migth be usefulindetection of MRD.

关 键 词：儿童 急性 T细胞 白血病 基因缺失 tal-1基因 

分 类 号：R733.710.4[医药卫生—肿瘤]