先天性中胚层肾瘤的流式细胞术分析及其与肾母细胞瘤的比较  

Flow Cytometric Analysis of Congenital Mesoblastic Nephroma and Nephroblastoma

作　　者：周晓岩[1] 王琳[1] 冯畹兰[1] 

机构地区：[1]白求恩医科大学第一临床学院小儿外科

出　　处：《中华小儿外科杂志》1995年第5期284-285,共2页Chinese Journal of Pediatric Surgery

基　　金：吉林省应用基础研究资助

摘　　要：应用流式细胞术对５例先天性中胚层肾瘤、９６例肾母细胞瘤和２７例正常肾石蜡包埋组织进行了ＤＮＡ含量和增殖活性分析。同时进行组织学观察。结果表明，无论在出现非整倍体细胞峰上，还是ＤＩ值、Ｓ％、ＰＩ值上，先天性中胚层肾瘤与肾母细胞瘤比较均有显著性差异。由此提出了肾母细胞瘤流１式诊断标准与先天性中胚层肾瘤相鉴别。同时分析了临床造成误诊的主要原因。结合随访认为对出现ＤＮＡ非整倍体的先天性中胚层肾瘤以单纯切除及定期复查为最好。The DNA content and cell cycle in paraffin-embedded sections collected from 5 cases of congenital mesoblastic nephroma.96 cases of nephroblastoma and 27 normal kidneys were studied by flow cytometry.All sections were reviewed by a pathologist unknowing the outcome of DNA analysis.The results indicated that DNA index,proliferative index and the number of S phase cell were significantly different between congenital mesoblastic nephroma and nephroblastoma besides a distinct aneuploid stem cell line.Accordingly,a diagnostic criterion of flow cytometry for nephroblastoma is proposed.Congenital mesoblastic nephroma could be distinguished from nephroblastoma by this criterion.The main reason of clinical misdiagnosis is discussed.For congenital mesoblastic nephroma with DNA aneuploid,simple resection and regular follow up are advisable.

关 键 词：中胚层肾瘤 肾母细胞瘤 流式细胞仪 病理 肾肿瘤 

分 类 号：R737.11[医药卫生—肿瘤]