白细胞介素6基因转移的白血病细胞体内诱导小鼠免疫功能的实验研究  

EXPERIMENTAL STUDY ON THE IN Vlvo INDUCTONOF IMMUNE FUNCTION BY IL-6 GENE-TRANSFECTEDMOUSE LEUKEMIA CELLS

作　　者：葛林阜[1] 曹雪涛[1] 于益芝[1] 郑玲莉[1] 周正芳[1] 徐志工[1] 

机构地区：[1]第二军医大学免疫学教研室,济南军区总医院血液科

出　　处：《中华血液学杂志》1995年第6期300-303,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金;上海科技启明星计划

摘　　要：作者观察了人白细胞介素６（ＩＬ－６）基因转移的、能高分泌ＩＬ－６的ＦＢＬ－３红白血病细胞对机体抗肿瘤免疫功能的影响。结果发现，高分泌ＩＬ－６的红白血病细胞接种到小鼠体内后，能显著提高小鼠脾脏的细胞毒性Ｔ淋巴细胞、ＮＫ活性及ＩＬ－２诱导的ＬＡＫ活性，小鼠脾细胞诱生的ＩＬ－２、肿瘤坏死因子和粒－巨噬细胞系集落刺激因子水平亦增高，腹腔巨噬细胞杀伤活性明显增强。结果表明，高分泌ＩＬ－６的ＦＢＬ－３红白血病细胞体内接种后能显著地增强机体的抗肿瘤免疫功能。研究结果为白血病的生物治疗以及ＩＬ－６基因转移的新型瘤苗的应用研究提供了实验依据。n order to investigate the anti-leukemia effect ofthe tumor vaccine, we transfected the mouse FBL-3leukemia cell with IL-6 gene and selected a clone se-creting the highest level of IL-6 . It was found that af-ter inoculation of the IL-6 secreting cell clone into themouse, its CTL, NK and IL-2 induced LAK activitieswere all significantly augmented. Furthermore, thelevels of IL-2, TNF and GM-CSF secreted by murinesplenocyte were also increased , and the cytotoxicity ofperitoneal macrophage was significantly augmented.These results suggested that inoculation of IL-6 secret-ing leukemia cells can effectively enhance the host anti-leukemia immunological functions.

关 键 词：白细胞介素6 基因治疗 白血病 细胞因子 

分 类 号：R733.7[医药卫生—肿瘤]