视网膜增殖性疾病玻璃体中胰岛素样生长因子的放射免疫测定  

Significance and detection of insulin-like growth factor I in vitreous with proliferative retinal diseases by radioimmunoassay

作　　者：曹景泰[1] 武丽[1] 张惠蓉[1] 刘宁朴[1] 贺师鹏[1] 陈非[1] 

机构地区：[1]北京医科大学第三医院眼科,石家庄白求恩国际和平医院临床实验科,北京医科大学基础医学院生物物理系,北京医科大学基础医学院免疫系

出　　处：《中华眼科杂志》1995年第2期122-125,共4页Chinese Journal of Ophthalmology

摘　　要：用放射免疫分析测定正常人眼和各种视网膜增殖性疾病玻璃体中的胰岛素样生长因子（ｉｎｓｕｌｉｎ－ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒＩ，ＩＧＦ－Ｉ）水平共６７例。测前用酸酒精提取法去除ＩＧＦ结合蛋白。结果显示：各疾病组玻璃体ＩＧＦ－Ｉ水平较对照组有不同程度升高。增殖性糖尿病视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｄｉａｂｅｔ－ｉｃｒｅｔｉｎｏｐａｔｈｙ，ＰＤＲ）组和视网膜中央静脉阻塞（ｃｅｎｔｒａｌｒｅｔｉｎａｌｖｅｉｎｏｃｃｌｕｓｉｏｎ，ＣＲＶＯ）组的ＩＧＦ－Ｉ水平显著高于对照组。ＰＤＲ组玻璃体ＩＧＦ－Ｉ水平升高，与其血清浓度增高呈显著正相关。玻璃体内ＩＧＦ－Ｉ水平增高可能在视网膜增殖疾病发生发展中起重要作用。我们对照了提取结合蛋白后与自然玻璃体直接测定的差异，结合蛋白显著影响ＩＧＦ－Ｉ的测定。The insulin-like growth factor I(IGF-I)levels in 67 vitreous samples taken from 57 pa-tients with various proliferative retinal diseases and 10 normal subjects were detected by radioim-munoassay(RIA).The acid-ethanol-extracted(AEE)method was used to remove the IFG-binding protein before the detection.Our results revealed that IGF-I levels were increased to varying extents in these patients’vitreous.The IGF-I levels in the vitreous with proliferative diabetic retinopathy(PDR)and central retinal vein occlusion(CRVO)were significantly higher than those of the controls.In the PDR group,the increased IGF-I level in the vitreous was positively correlated with the level in serum.The increased IGF-I level in vitreous may play an important role in the development and pathogenesis of proliferative retinal diseases. Great differences were found between the native and the AEE treated vit-reous,suggesting that the binding protein posed an obvious interference on the detection of IGF-I by RIA.

关 键 词：胰岛素样 生长因子 玻璃体 视网膜疾病 放射免疫 

分 类 号：R774.120.2[医药卫生—眼科]