脲酶阳性副溶血弧菌的临床及实验研究  

Clinical and experimental research of urease positive vibrio parahaemolyticus.

作　　者：黄上媛[1] 刘永福[1] 周志江[1] 张维国[1] 

机构地区：[1]解放军第三○二医院微生物研究室,解放军北京医学高等专科学校,解放军农牧大学

出　　处：《中华医学检验杂志》1995年第2期84-86,共3页

摘　　要：从１４２９例急性腹泻患者粪便中分离出４２株副溶血弧菌，其中脲酶阳性为１５株占３５．７％。４２株菌均产生耐热溶血素，不产生不耐热溶血素；生化特性除鸟氨酸外其它结果均相同；其中７株脲酶阳性与２株脲酶阴性株，经聚合酶链反应检测发现它们的扩增产物均具有相同分子量定位于４１６ｂｐ的荧光带，从而证实脲酶阳性株与脲酶阴性株一样都拥有ｔｄｈ基因。脲酶阳性株感染的１５例患者均有典型急性胃肠炎症状，因此在腹泻病原菌检测时不可忽视对这种新的副溶血弧菌致病株──脲酶阳性株的分离鉴定。2 strains of V. parahaemolyticus were isolated.from 1429 patients with acute diarrhea,all of them produced TDH hemolysin and no TRH hemolysin, 15 strains of them were urease positive(35.7%).42 strains had the same biochemical characterristics either urease positive or negative,except ornithine de-carboxlase, 7 urease positive and 2 urease negative strains were studied by PCR. It was shown that products had the same molcular weight and located at 416 bp of fluorescent band. So urease positive and negative stains had tdh genes. There were representative symptoms of acute gastroenteritis in the 15 cases who were infected with urease positive V. parahaemolyticus. Therefore,we should pay attention to isolate and identify urease positive V, parahaemolyticus while detecting the pathogenic of diarrhea.

关 键 词：副溶血弧菌 脲酶 微生物检验 

分 类 号：R446.5[医药卫生—诊断学]