应用荧光原位杂交技术在人肺癌中发现c－myc基因易位  

Detection of c-inyc translocation in human lung cancercells by FISH

作　　者：吕永杰[1] 程书钧[1] 郭素萍[1] 徐丽华[1] 董向阳[1] 

机构地区：[1]中国医学科学院肿瘤研究所病因室

出　　处：《中华医学杂志》1995年第4期214-215,共2页National Medical Journal of China

摘　　要：基因易位是癌基因激活的一种机制，但是以往检测基因改变的方法较难检测基因易位。我们建立了荧光原位杂交（ＦＩＳＨ）技术，井应用这项技术在人肺癌细胞系ＧＬＣ－８２和１例经ＳＶ４０Ｔ转化了的人支气管上皮细胞中发现了癌基因ｃ－ｍｙｃ易位。在ＧＬＣ－８２细胞系中ｃ－ｍｙｃ基因易位到一条Ｃ组大小标记染色体的短臂。在ＳＶ４０Ｔ转化支气管上皮细胞中ｃ－ｍｙｃ易位到１４ｑ３２，提示ｃ－ｍｙｃ基因易位可能在肺癌起因中起重要作用。-myc gene amplification has been found in lungcancer, however, it can not explain all cases of lungcancer with c-myc gene overexpression. Gene transloca-tion is one of the ways by which oncogene is activated.But the old methods for detecting gene mutations arenot so effective for the detection of gene translocation,especially in solid tumors. Fluorescence in situ hy-bridization (FISH) can be used to detect gene transloca-tion more efficiently. Using FISH , we discovered c-mycgene translocation in a lung adenocarcinoma cell hneGLC-82 and SV40T-transformed human bronchial ep-ithelial cells. In GLC-82, c-myc gene translocated tothe short arm of a C group marker chromosome. In theSV40T-transformed epithelial cells , c-myc gene translo-cated to 14q32, which was the same as that found inBurkitt’s lymphomas. Translocation was related tooncogene activation. c-myc translocation may play animportant role in the carcinogenesis of lung cancer.

关 键 词：肺肿瘤 基因 DNA探针 病因 癌 

分 类 号：R734.212[医药卫生—肿瘤]