联合应用白介素2和白介素6基因转染瘤苗抗肿瘤的实验研究  

Antitumor effect of IL-2 gene-transfected tumor vaccine in combination with IL-6 gene-transfected tumor vaccine

作　　者：曹雪涛[1] 章卫平[1] 陶群[1] 于益芝[1] 陈国友[1] 叶天星[1] 

机构地区：[1]第二军医大学免疫学教研室

出　　处：《中华医学杂志》1995年第10期602-605,共4页National Medical Journal of China

基　　金：国家自然科学基金;军队八五科技攻关项目

摘　　要：为了提高细胞因子基因转染瘤苗治疗肿瘤的效果，本实验观察了联合应用白细胞介素２（ＩＬ－２）基因转染瘤苗和白细胞介素６（ＩＬ－６）基因转染瘤苗治疗黑色素瘤实验性肺转移小鼠的效果，结果表明，联合治疗组荷瘤小鼠的肺转移结节数显著地少于单用瘤苗治疗组，其存活期也显著长于单一瘤苗治疗组，其脾脏细胞毒性Ｔ淋巴细胞（ＣＴＬ）活性、天然杀伤细胞（ＮＫ）活性、淋巴因子激活的杀伤细胞（ＬＡＫ）活性以及ＩＬ－２和ＴＮＦ分泌水平均非常显著地高于单一瘤苗治疗组水平。实验结果说明，ＩＬ－２基因转染瘤苗与ＩＬ－６基因转染瘤苗联合应用后，能更有效地激活免疫功能达到更佳的抗肿瘤效果。It has been shown that IL-2 and IL-6 can cooperate to induce immune responses more effectively.In order to increase the therapeutic efficacy of cytokine genetransfected tumor vaccines,we treated the experimental pulmonary metastatic melanoma-bearing mice with IL-2 gene-transfected tumor vaccine and IL-6 gene-transfected tumor vaccine.After the combined treatment,the pulmonary metastases were reduced more significantly and the survival time of tumor-bearing mice was also prolonged more significantly. The CTL activity,NK activity and IL-2-induced LAK activity,IL-2 and TNF secretion from the splenocytes of the above tumor-bearing mice increased more significantly than that of tumorbearing mice treated with IL-2 gene-transfected vaccine or IL-6 gene-transfected vaccine alone. These results demonstrated that the combined use of IL-2 gene-transfected tumor vaccine and IL-6 gene-transfected tumor vaccine could achieve more potent antitumor effect via more efficient activation of immune functions.The experiment outlines a novel approach to the cytokine gene therapy of cancer.

关 键 词：肿瘤 免疫疗法 白细胞介素 基因转染 瘤苗 

分 类 号：R730.51[医药卫生—肿瘤] R979.19[医药卫生—临床医学]