抗人肝癌单克隆抗体对荷瘤裸鼠的放射免疫定位及治疗  

RADIOIMMUNOIMAGING(RII)AND RADI0IMMUNOTHERAPY(RIT)OF HUMAN PRIMARY HEPATIC CANCER(hPHC)WITH ANTI-PHC MON0CLONAL ANTIBODY IN TUMOR BEAING NUDE MICE

作　　者：施乐华[1] 吴孟超[1] 陈汉[1] 陶文照[1] 强美玉[1] 谢弘[1] 王根风[1] 王放[1] 

机构地区：[1]上海第二军医大学长海医院肝胆外科,第二军医大学病理解剖学教研室,中科院上海细胞生物学研究所

出　　处：《中华肿瘤杂志》1995年第1期20-23,共4页Chinese Journal of Oncology

摘　　要：以１３１－碘标记抗人肝癌单克隆抗体Ｈｅｐａｍａ－１（以下简称１３１－Ｈｅｐ）进行裸鼠人原发性肝癌（ＰＨＣ）的体内肿瘤定位（ＲＩＩ）及杀伤作用（ＲＩＴ）的研究。单抗系小鼠ＩｇＧ＿１，选用高效液相法纯化，Ｉｏｄｏｇｅｎ法标记１３１－ＮａＩ。行ＲＩＩ时，每鼠从尾静脉注射１３１－Ｈｅｐ１．１１×１０￣７Ｂｑ；行ＲＩＴ时，从腹腔注入１３１－Ｈｅｐ１．８５×１０￣１０Ｂｑ。结果：Ｈｅｐ能选择性地积聚于肿瘤组织，对肿瘤生长有明显的抑制作用，并能显著延长荷瘤鼠的生存时间。Abstract Tumor xenografts of human PHC were grown in nude mice subcutaneously.The studies wereperformed in nude mice with tumor base aear of 0.8cm～1.0cm.For the study of RII,the mice wereinjected intravenously with 1.11×10￣ 7Bq/mouse of ￣131I-hepamal-1.All tumours were clearly visualiedon the 4th day after injection and remained the same until the 7th day.No tumor image was found in￣131I-mIgG.The tumor to liver ratio of radioactivity increased from 1.3 to 3.03 in￣ 131I-Hepama-1group,but decreased from 13.6 to 6.88%in ￣131I-mIgG group.For the study of RIT￣131I-hepama-1(1.85 ×10￣10Bq)was administered intraperitoneally.A tumor grouth inhibition rate at the 4th weekafter the infusion of 70.5%was obtained.The median survival time of the nude mice in￣131I-hepama-1 group was much longer than in control groups of mice.

关 键 词：肝肿瘤 单克隆抗体 放射免疫测定 免疫疗法 

分 类 号：R735.705[医药卫生—肿瘤]