人乳腺癌原代培养体外药敏试验的评价  

EVALUATION OF CHEMOSENSITIVITIES OF HUMAN BREAST CANCERS BY PRIMARY CULTURE ASSAY IN VITRO

作　　者：徐建明[1] 汤仲明[1] 宋三泰[1] 江泽飞[1] 李家益 杨奕静[1] 

机构地区：[1]北京军事医学科学院附属医院

出　　处：《中华肿瘤杂志》1995年第2期100-103,共4页Chinese Journal of Oncology

摘　　要：作者采用ＭＴＴ比色法，测定了３２例体外原代乳癌细胞对６种常用化疗药物的敏感性。结果表明，不同乳癌对同一化疗药物的敏感性不同，不同乳癌有其各自的药敏谱。由于本组瘤群的ＩＣ＿５０＿呈明显的偏态分布（Ｐ＜０．０００１），按ＩＣ＿５０的大小，可用百分位数法将其敏感性分为四类。初步临床分析表明，不同化疗药物的体外敏感性与治疗效果基本相符，提示本分析法可作为优化个体化疗的有效途径。AbstractChemosensitivities of 32 human breast cancers to 6 antitumor drugs were assayed in vitrousing MTT colorimetric method.The results showed that the chemosensitivity of primary breastcancer cultures varied remarkably from one patient,and from one drug,to another with hun-dred-to thousand-fold differences.The distribution of 50% inhibition concentrations (IC_50)was significantly deviated from normal distribution(P<0.0001), and the drug sensitivity couldbe divided into four different catagories by means of percentile method. Preliminary resultsshowed that the in vitro chemosensitivities of different antitumor drugs were consistent with theclinical therapeutic responses It seems that the herein reported assay might be useful on an indi-vidual basis to optimize chemotherapy of human breast cancer.

关 键 词：乳腺癌 肿瘤细胞 体外培养 药敏试验 

分 类 号：R73－36[医药卫生—肿瘤] R737.905[医药卫生—临床医学]