二乙基亚硝胺诱发大鼠肝癌前病变中c－myc、N－ras和γ－GT酶表达的相关性研究  

A STUDY ON THE RELATIONSHIP BETWEEN THE EXPRESSION OF c-myc,N-ras ONCOGENES ANDγ-GT ASE IN PRECANCEROUS CHANGE OF RAT LIVER

作　　者：郑杰[1] 武忠弼[1] 阮幼冰[1] 刘冰[1] 

机构地区：[1]同济医科大学超微病理研究室,北京中国医学科学院肿瘤研究所分子肿瘤国家重点实验室

出　　处：《肿瘤》1995年第6期462-465,共4页Tumor

摘　　要：本文应用ｃＤＮＡ－ＲＮＡ原位杂交技术和组织化学方法，观察了二乙基亚硝胺（ＤＥＮＡ）诱发大鼠肝癌前病灶中ｃ－ｍｙｃ、Ｎ－ｒａｓ癌基因和γ－ＧＴ酶的表达。结果表明，ｃ－ｍｙｃ、Ｎ－ｒａｓ的过度表达出现于早期增生的小肝细胞以及变异肝细胞灶、增生结节和腺瘤中，γ－ＧＴ酶出现稍晚（诱癌６周），起初在病变中的分布与癌基因相似，而在诱癌１２周后，酶改变病灶数较癌基因要多。提示两者表达有相关住，癌基因激活可能诱导γ－ＧＴ酶的表达，而且一旦γ－ＧＴ基因启动后，则可能不受癌基因活动的影响，同时也表明，并非所有γ－ＧＴ酶异常细胞都具有癌变倾向。Using cDNA-RNA in situ hybridization technique and histochemical method,the expression of c-myc,N-ras and γ-GT ase in precancerous change of rat liver induced by diethylnitrosamine was observed.The expression of c-myc,N-ras was enhanced in foci of proliferated small hepatocytes and altered hepatocytes as well as hyperplastic nodules.The γ-GT ase appeared later (6 weeks after DENA administration),and showed a similar distribution as oncogenes.However,after 12 weeks exposed to DENA,the γ-GT ase positive foci was more than that of c-myc's and N-ras.These results indicated that the expression of c-myc and N-ras genes in the lesions appeared to be related to the cellular proliferation status and to the reappearance of γ-GT ase.It might suggest that the overexpression of c-myc and N-ras genes could induce the expression of γ-GT gene,Furthermore,once the γ-GT gene was activated,it's expression would not be influenced by the oncogenes.

关 键 词：癌基因 γ-GT酶 肝癌前病变 DENA 

分 类 号：R735.702[医药卫生—肿瘤]