用DABS—CL试剂柱前衍生技术和反相HPLC法分离人血清中游离氨基酸  

SEFARATION OF FREE AMINO ACIDS IN HUMAN SERUM BY HPLC WITH DABS - CL PRE - COLUMU DERIVATIZATION METHOD

作　　者：江国荣[1] 费达[2] 

机构地区：[1]苏州市中医药研究所 [2]苏州大学分析测试中心

出　　处：《苏州大学学报（自然科学版）》1995年第3期65-68,共4页Journal of Soochow University(Natural Science Edition)

摘　　要：本文报导了用DAB5—CL柱前衍生HPLC法,在室温条件下,直接分离人血清中十八种游离基酸。对可能影响血清中游离氮基酸分离的一些因素作了探讨。结果表明该衍生技术较为迅速,简单,衍生物(DABS—氨基酸)具有较高的稳定性和灵敏度,整个衍生过程所需一小时内完成在25℃左右,血清氨基酸可得到较为满意的分离。Here we report that,with the DABS - CL pre - column derivatization technique, the free amino acids in human serum are easily separated by reverse phase liquid chromatography at room temperature. Some of the factors which probobly interfere with the separation of free aminoacids in human serum are disccussed. The results show that the DABS - CL derivatiaztion is highly stable and sensitive, the derivatization procedure is relatively simple and fairly rapid,the whole procedure takes less than one hour. The temperature should be fixed at about 25℃ in order to obtain more satisfactory seperature of free amino acids in human serum.

关 键 词：高效液相色谱 血清 氨基酸 DABS-CL 分离 

分 类 号：R446.112[医药卫生—诊断学]