肝癌及癌旁组织丙肝病毒基因负链研究  

Study of the Minus Strand of HCV-RNA in Tumor and Pericancerous Nontumor Liver Tissue in Patients with Hepatocellular Carcinoma

作　　者：查文章[1] 杜竞辉[1] 王学浩[1] 黄祖湖 李军[1] 

机构地区：[1]南京医学院肝癌研究中心,盐城市第一人民医院

出　　处：《中国肿瘤临床》1995年第3期171-174,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金

摘　　要：报告运用巢式ＰＣＲ检测５例ＨＣＶ－ＲＮＡ阳性肝癌及癌旁组织和周围血清中ＨＣＶ－ＲＮＡ负链．正链检测：血清及癌旁组织全部阳性，癌组织中３例阳性；负链检测：血清中均阴性，除２例癌组织外癌旁组织５例均阳性。巢式ＰＣＲ是检测ＨＣＶ感染病人外周血中ＨＣＶ－ＲＮＡ非常敏感的一种方法。有助于区别病毒基因是来源于组织本身还是血液中ＨＣＶ粘附于组织上。在肝癌组织中检测到ＨＣＶ－ＲＮＡ负链，推测ＨＣＶ在其中复制，持续感染，有可能构成致癌作用的启动因子，对肝癌的发生起了一定的作用。Sera and tissue obtained from patients with hepatocellular carcinoma were examined for the presence of hepatitis C virus (HCV) genome by nested polymerase chain reaction (PCR) assay.Whereas plus-stranded HCV-RNA was detectable in sera,hepatocellular carcinoma and pericancerous nontumor liver tissue of type C chronic liver disease Patients,the minus-stranded HCV-RNA was only detectable in the liver tissue.Nested-PCR is a very powerful tool for the detection of HCV-RNA in the sera of patients with HCV infection.It assists in distinguishing between blood viral particles adhering to the tissue and viral RNA contained within the tissue.Detection of the minus-strand of hepatitis C virus RNA by nested-PCR may be implicated in hepatitis C virus replication in infected tissue.This virus may not only function as initiators of hepatocarcinogenesis but may also as a promoter of carcinogenesis.

关 键 词：丙型肝炎病毒 肝细胞癌 聚合酶链反应 基因 负链 

分 类 号：R735.703[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]