地奥心血康对心肌缺血再灌犬心肌细胞膜Na~＋，K~＋—ATP酶活性与LPO含量  

Effects of DAXXK on Na￣+,K￣+-ATPase activity and LPO(lipid peroxides)content of myocardial cell membranes in myocardial reperfusion injury dogs

作　　者：刘睿智[1] 李扬[1] 赵丹[1] 刘子红[1] 王蔚东[1] 赵雪俭[1] 吕文伟[1] 陈羽[1] 张键[1] 郭新雯[1] 周正质[1] 刘中荣[1] 

机构地区：[1]长春白求恩医科大学基础医学院病理生理研究室,长春白求恩医科大学生理中心实验室,长春白求恩医科大学第一临床医院心血管内科,中国科学院成都地奥制药集团

出　　处：《中国病理生理杂志》1995年第4期409-412,共4页Chinese Journal of Pathophysiology

摘　　要：１６只成年杂种犬复制心肌缺血再灌模型。分为地奥心血康治疗组（ＤＡＸＸＫＧ）和生理盐水对照组（ＮＳＣＧ）。差速离心分离心肌细胞质膜，无机磷法测定心肌细胞膜Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性，荧光法测定心肌细胞膜与血清脂质过氧化物（ＬＰＯ）含量。结果表明：（１）ＤＡＸＸＫＧＮａ￣＋，Ｋ￣＋－ＡＴＰ酶活性明显高于ＮＳＣＧ（Ｐ＜０．０１）；（２）血清ＬＰＯ含量，随着再灌时间延长，ＮＳＣＧ呈上升趋势，ＤＡＸＸＫＧ略下降，再灌２４０ｍｉｎ两组比较差异显著（Ｐ＜０．０１）；心肌细胞膜ＬＰＯ含量，ＤＡＸＸＫＧ低于ＮＳＣＧ（Ｐ＜０．０５）；（３）心肌细胞膜Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性与ＬＰＯ含量呈明显负相关（ｒ＝－０．８３，Ｐ＜０．０１）。这些结果提示ＤＡＸＸＫ可通过抗脂质过氧化而实现其保护心肌细胞膜的效应。Sixteen adult hybrid dogs induced myocardial reperfusion injury wererandomly divided into Di’Ao-Xin-Xue-Kang(DAXXK) treated group and normal saline(NS)control group. Myocardial cell membranes were seperated by different centrifugalspeed method.Na￣+,K￣+-ATPase activity was measured by inorganic phospliate determina-tion,LPO content of myocardial cell membranes and serum was measured according tofluorescence method. The results indicated that:Na￣+,K￣+-ATPase activity of DAXXKgroup was much higher than that of NS group ( P<0).②The more is the time ofreperfusion, the higher is the content of LPO in NS group. There was a reducing ten-dency in DAXXK group. At 240 min of reperfusion there was a significant differencebetween DAXXK group and NS group( P<0.01).LPO content of myocatdial cell mem-branes in DAXXK group was lower than that in NS group( P<0.05).③Myocardialcell membranes Na￣+,K￣+-ATPase activity was closely related to LPO content(r=-0.83,P<0.01)These results showed that the mechanism of the protecting effect of DAXXKon ischemia reperfused myocardium might be associated with the inhibition of cellularlipid peroxidation.

关 键 词：心肌 细胞膜 脂质过氧化物 地奥心血康 心肌缺血 

分 类 号：R363.13[医药卫生—病理学] R969.4[医药卫生—基础医学]